Literature summary for 3.4.24.40 extracted from

Dahler, G.S.; Barras, F.; Keen, N.T.
Cloning of genes encoding extracellular metalloproteases from Erwinia chrysanthemi EC16 (1990), J. Bacteriol., 172, 5803-5815.

Search for more literature for 3.4.24.40

Cloned(Commentary)

Cloned (Comment)	Organism
Erwinia chrysanthemi	Dickeya chrysanthemi
expressed in Escherichia coli	Dickeya chrysanthemi

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	-	Dickeya chrysanthemi
Periplasmic endogen inhibitor peptide of Serratia marcescens	-	Dickeya chrysanthemi

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Dickeya chrysanthemi	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	activation	Dickeya chrysanthemi
Mg2+	activation	Dickeya chrysanthemi
additional information	no activation by Rb2+	Dickeya chrysanthemi
Zn2+	zinc-containing metalloproteinase (not)	Dickeya chrysanthemi

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	Erwinia chrysanthemi, wild-type enzyme or recombinant protease C, SDS-PAGE	Dickeya chrysanthemi
51100	-	Erwinia chrysanthemi, recombinant protease C, calculated from nucleotide sequence	Dickeya chrysanthemi

Organism

Organism	UniProt	Comment	Textmining
Dickeya chrysanthemi	-	plant pathogen	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Gelatin + H2O	-	Dickeya chrysanthemi	?	-	?
hide powder azure + H2O	-	Dickeya chrysanthemi	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 50000, Erwinia chrysanthemi, wild-type enzyme or recombinant protease C, SDS-PAGE, x * 50000-52500, Erwinia chrysanthemi, 3 recombinant proteases A-C, SDS-PAGE	Dickeya chrysanthemi

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
additional information	-	pI: 5.25 (calculated value)	Dickeya chrysanthemi

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Release 2023.1 (January 2023)