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Literature summary for 3.4.24.40 extracted from

  • Chessa, J.P.; Petrescu, I.; Bentahir, M.; Van Beeumen, J.; Gerday, C.
    Purification, physico-chemical characterization and sequence of a heat labile alkaline metalloprotease isolated from a psychrophilic Pseudomonas species (2000), Biochim. Biophys. Acta, 1479, 265-274.
    View publication on PubMed
Search for more literature for 3.4.24.40

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Pseudomonas sp.
EDTA 10 mM, pH 8, strong inhibition Pseudomonas sp.
EGTA 10 mM, pH 8, strong inhibition Pseudomonas sp.
HgCl2 complete inhibition Pseudomonas sp.
additional information not inhibited by dithiothreitol, PMSF and DFP Pseudomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pseudomonas sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stabilizing Pseudomonas sp.
Zn2+
-
 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48690
-
 electrospray mass spectrometry Pseudomonas sp.
48690
-
 calculated Pseudomonas sp.
48730
-
 electrospray mass spectrometry Pseudomonas sp.
48770
-
 electrospray mass spectrometry Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. TAC-II-18
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 113 U/mg, one unit gives an increase of 0.01 absorbance at 366 nm, excess of azocasein in 20 mM Tris, 1 mM CaCl2, pH 8 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Pseudomonas sp. ?
-
 ?
azocasein + H2O
-
 Pseudomonas sp. TAC-II-18 ?
-
 ?

Synonyms

Synonyms Comment Organism
psychrophilic alkaline metalloprotease PAP Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4
-
 highest acitivity after 4 days of culture Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 50% activity after 20 min Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 10
-
 Pseudomonas sp.