3.4.22.B79: nsP2 protease

This is an abbreviated version!
For detailed information about nsP2 protease, go to the full flat file.

Reaction

the enzymes processes the alphavirus nonstructural polyprotein (nsP1234). The enzyme from Venezuelan equine encephalitis virus shwos a preferens for Gly or Als in position P1', Ala or Cys in P1, and Gly in P2 =

Synonyms

non-structural polyprotein 2, nonstructural polyprotein, nonstructural protein, nonstructural protein 2, ns polyprotein, nsP2, nsP2 protease, nsp2 protein, nsp2pro, p39 nsp2 protease

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.22 Cysteine endopeptidases

                3.4.22.B79 nsP2 protease

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
2	Activating Compound
11	AA Sequence
12	Application
13	Cloned(Commentary)
10	Crystallization (Commentary)
9	General Information
18	Inhibitors
23	kcat/KM [mM/s]
35	KM Value [mM]
3	Localization
3	Metals/Ions
2	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
51	Organism
1	pH Optimum
2	pH Range
2	Posttranslational Modification
81	Protein Variants
6	Purification (Commentary)
1	Reaction
45	Reference
1	Storage Stability
37	Substrates and Products (Substrate)
4	Subunits
24	Synonyms
3	Temperature Optimum [°C]
2	Temperature Range [°C]
3	Temperature Stability [°C]
30	Turnover Number [1/s]

Substrates Products

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Substrates Products on EC 3.4.22.B79 - nsP2 protease

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to Substrate/Product Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

2-(N-methylamino)benzoyl-AGAGIIETk(Dnp) + H2O

2-(N-methylamino)benzoyl-AGA + GIIETk(Dnp)

3 entries

2-(N-methylamino)benzoyl-AGCGIIETk(Dnp) + H2O

2-(N-methylamino)benzoyl-AGC + GIIETk(Dnp)

3 entries

2-(N-methylamino)benzoyl-AGGGIIETk(Dnp) + H2O

2-(N-methylamino)benzoyl-AGG + GIIETk(Dnp)

3 entries

4-(4-dimethylaminophenyl-azo)benzoyl-DRAGGYIFSE-Edans + H2O

4-(4-dimethylaminophenyl-azo)benzoyl-DRAGG + YIFSE-Edans

2 entries

4-[[4-(dimethylamino)phenyl]-azo]benzoyl-DELRLDRAGGYIFSS-Edans + H2O

4-[[4-(dimethylamino)phenyl]-azo]benzoyl-DELRLDRAGG + YIFSS-Edans

2 entries

4-[[4-(dimethylamino)phenyl]-azo]benzoyl-LDRAGGYI-Edans + H2O

4-[[4-(dimethylamino)phenyl]-azo]benzoyl-LDRAGG + YI-Edans

2 entries

DABCYL-Arg-Ala-Gly-Gly-Tyr-Ile-Phe-Ser-(Glu-EDANS) + H2O

DABCYL-Arg-Ala-Gly-Gly + Tyr-Ile-Phe-Ser-(Glu-EDANS)

Chikungunya virus

C7S7A0

-

755486

-

-

?

MBPNusG-His6 p3/p4 + H2O

?

Semliki forest virus

-

-

701065

-

-

?

P123 polyprotein + H2O

?

Sindbis virus

-

-

699903

-

-

?

P23 polyprotein + H2O

?

Sindbis virus

-

-

699903

-

-

?

Semliki forest virus p1/p2 + H2O

?

2 entries

Semliki forest virus p3/p4 + H2O

?

Semliki forest virus

-

-

701065

-

-

?

Sindbis virus core protein + H2O

Hydrolyzed Sindbis core protein

Sindbis virus

-

-

29849, 681688

-

-

?

thioredoxin fusion protein + H2O

?

Semliki forest virus

-

fusion protein is cleaved with greater efficiency by nsp2pro than the original MBP-NusG-His6 substrate with the shorter linker

701065

-

-

?

Trx34 + H2O

?

Semliki forest virus

-

-

679819

-

-

?

Venezuelan equine encephalitis virus p1/p2 + H2O

?

Venezuelan equine encephalitis virus

P27282

-

701065

-

-

?

Venezuelan equine encephalitis virus p3/p4 + H2O

?

Venezuelan equine encephalitis virus

P27282

-

701065

-

-

?

XJ-160 virus-specific protein + H2O

?

Sindbis virus

-

-

710679

-

-

?

additional information

?

-

8 entries

2-(N-methylamino)benzoyl-AGAGIIETk(Dnp) + H2O

2-(N-methylamino)benzoyl-AGA + GIIETk(Dnp)

Chikungunya virus

-

-

755412

-

-

?

2-(N-methylamino)benzoyl-AGAGIIETk(Dnp) + H2O

2-(N-methylamino)benzoyl-AGA + GIIETk(Dnp)

Chikungunya virus

-

corresponds to cleavage site P1/2 of the polyprotein

752809

-

-

?

2-(N-methylamino)benzoyl-AGAGIIETk(Dnp) + H2O

2-(N-methylamino)benzoyl-AGA + GIIETk(Dnp)

Chikungunya virus ECSA E1:226V

-

-

755412

-

-

?

2-(N-methylamino)benzoyl-AGCGIIETk(Dnp) + H2O

2-(N-methylamino)benzoyl-AGC + GIIETk(Dnp)

Chikungunya virus

-

-

755412

-

-

?

2-(N-methylamino)benzoyl-AGCGIIETk(Dnp) + H2O

2-(N-methylamino)benzoyl-AGC + GIIETk(Dnp)

Chikungunya virus

-

corresponds to cleavage site P2/3 of the polyprotein

752809

-

-

?

2-(N-methylamino)benzoyl-AGCGIIETk(Dnp) + H2O

2-(N-methylamino)benzoyl-AGC + GIIETk(Dnp)

Chikungunya virus ECSA E1:226V

-

-

755412

-

-

?

2-(N-methylamino)benzoyl-AGGGIIETk(Dnp) + H2O

2-(N-methylamino)benzoyl-AGG + GIIETk(Dnp)

Chikungunya virus

-

-

755412

-

-

?

2-(N-methylamino)benzoyl-AGGGIIETk(Dnp) + H2O

2-(N-methylamino)benzoyl-AGG + GIIETk(Dnp)

Chikungunya virus

-

corresponds to cleavage site P3/4 of the polyprotein

752809

-

-

?

2-(N-methylamino)benzoyl-AGGGIIETk(Dnp) + H2O

2-(N-methylamino)benzoyl-AGG + GIIETk(Dnp)

Chikungunya virus ECSA E1:226V

-

-

755412

-

-

?

4-(4-dimethylaminophenyl-azo)benzoyl-DRAGGYIFSE-Edans + H2O

4-(4-dimethylaminophenyl-azo)benzoyl-DRAGG + YIFSE-Edans

Chikungunya virus

Q8JUX6

-

753844

-

-

?

4-(4-dimethylaminophenyl-azo)benzoyl-DRAGGYIFSE-Edans + H2O

4-(4-dimethylaminophenyl-azo)benzoyl-DRAGG + YIFSE-Edans

Chikungunya virus S27-African prototype

Q8JUX6

-

753844

-

-

?

4-[[4-(dimethylamino)phenyl]-azo]benzoyl-DELRLDRAGGYIFSS-Edans + H2O

4-[[4-(dimethylamino)phenyl]-azo]benzoyl-DELRLDRAGG + YIFSS-Edans

Chikungunya virus

Q1W368

-

755448

-

-

?

4-[[4-(dimethylamino)phenyl]-azo]benzoyl-DELRLDRAGGYIFSS-Edans + H2O

4-[[4-(dimethylamino)phenyl]-azo]benzoyl-DELRLDRAGG + YIFSS-Edans

Chikungunya virus ECSA

Q1W368

-

755448

-

-

?

4-[[4-(dimethylamino)phenyl]-azo]benzoyl-LDRAGGYI-Edans + H2O

4-[[4-(dimethylamino)phenyl]-azo]benzoyl-LDRAGG + YI-Edans

Chikungunya virus

Q1W368

peptide representing the 1/2 site of the polyprotein. Substrate is processed by nsP2, but the reaction velocity cannot be saturated

755448

-

-

?

4-[[4-(dimethylamino)phenyl]-azo]benzoyl-LDRAGGYI-Edans + H2O

4-[[4-(dimethylamino)phenyl]-azo]benzoyl-LDRAGG + YI-Edans

Chikungunya virus ECSA

Q1W368

peptide representing the 1/2 site of the polyprotein. Substrate is processed by nsP2, but the reaction velocity cannot be saturated

755448

-

-

?

Semliki forest virus p1/p2 + H2O

?

Semliki forest virus

-

-

701065

-

-

?

Semliki forest virus p1/p2 + H2O

?

Venezuelan equine encephalitis virus

P27282

-

701065

-

-

?

additional information

?

-

Chikungunya virus

-

chikungunya nsP2 protease possesses different substrate specificity to the canonical alphavirus nsP2 polyprotein cleavage specificity

755412

-

-

?

additional information

?

-

Chikungunya virus

Q1W368

protease readily cleaves GFP-10:5-Trx substrates containing short P10-P5' sequences originating from the 1/2 and 3/4 cleavage sites of the polyprotein, but not from the 2/3 site. Enzyme prefers the 3/4 substrate for cleavage in trans

755448

-

-

?

additional information

?

-

Chikungunya virus ECSA

Q1W368

protease readily cleaves GFP-10:5-Trx substrates containing short P10-P5' sequences originating from the 1/2 and 3/4 cleavage sites of the polyprotein, but not from the 2/3 site. Enzyme prefers the 3/4 substrate for cleavage in trans

755448

-

-

?

additional information

?

-

Chikungunya virus ECSA E1:226V

-

chikungunya nsP2 protease possesses different substrate specificity to the canonical alphavirus nsP2 polyprotein cleavage specificity

755412

-

-

?

additional information

?

-

Sindbis virus

-

the catalytic dyad is composed of Cys-481 and His-558

29849

-

-

?

additional information

?

-

Sindbis virus

-

enzyme is critically involved in development of cytopathic effect. Cytotoxic effect of enzyme is due to its ability to cause transcriptional shutoff

666194

-

-

?

additional information

?

-

Sindbis virus

-

after negative-strand synthesis, the ns protein appears to irreversibly change conformation and forms mature replication complexes, in spite of the lack of ns polyprotein cleavage. Complete processing of the ns polyprotein is not an absolute prerequisite for Sindbis virus replication. Inability of the viruses with unprocessed polyprotein to productively replicate in the interferon-competent cells and in the cells of mosquito origin is an additional, important factor in ns polyprotein cleavage development. It leads to the release of nsP2 protein, which plays a critical role in inhibiting the cellular antiviral response

699901

-

-

?

additional information

?

-

Sindbis virus

-

recombinant Sindbis viruses encoding nsP2/GFP chimeric protein are capable of growth in tissue culture and interfering with cellular functions. GFP insertion after the eighth amino acid of nsP2 in the SIN/nsP2GFP/8 virus blocks the processing of one of the cleavage sites. In the SIN/nsP2GFP/472 virus, the GFP insertion causes the formation process for nsP2/GFP to pass through an additional step of processing, in which the intermediate product of a higher molecular weight is initially formed and then processed to a final form of nsP2/GFP

699903

-

-

?