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Literature summary for 3.4.22.B79 extracted from
- Role for conserved residues of sindbis virus nonstructural protein 2 methyltransferase-like domain in regulation of minus-strand synthesis and development of cytopathic infection (2008), J. Virol., 82, 7284-7297.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | SINV nsP2 is an important factor in viral RNA replication and modification of cell biology | Sindbis virus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| GFP-encoding DNA fragment randomly cloned into the nsP2 gene. Entire library of the recombinant nsP2/GFP genes transferred into wild-type SINV Toto1101 genome. BHK-21 cells infected with SIN/nsP2GFP/8 and with both packaged replicons and SIN/nsP2GFP/472 virus | Sindbis virus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G8063V | mutation blocks P2/3 cleavage during P123 processing | Sindbis virus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | SIN/nsP2GFP/8-specific fusion protein is present in the cytoplasm in large complexes, and very little is found in the nuclei, nsP2/GFP of SIN/nsP2GFP/472 is distributed both in the cytoplasm and in the nucleus | Sindbis virus | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sindbis virus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | recombinant Sindbis viruses encoding nsP2/GFP chimeric protein are capable of growth in tissue culture and interfering with cellular functions. GFP insertion after the eighth amino acid of nsP2 in the SIN/nsP2GFP/8 virus blocks the processing of one of the cleavage sites. In the SIN/nsP2GFP/472 virus, the GFP insertion causes the formation process for nsP2/GFP to pass through an additional step of processing, in which the intermediate product of a higher molecular weight is initially formed and then processed to a final form of nsP2/GFP | Sindbis virus | ? | - |
? |  |
| P123 polyprotein + H2O | - |
Sindbis virus | ? | - |
? | |
| P23 polyprotein + H2O | - |
Sindbis virus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nonstructural protein | - |
Sindbis virus |
| nsP2 | - |
Sindbis virus |
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