3.4.22.B79: nsP2 protease

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For detailed information about nsP2 protease, go to the full flat file.

Word Map on EC 3.4.22.B79

3.4.22.B79

polyproteins

viruses

alphavirus

chikungunya

replicase

sindbis

chikv

nsp1

semliki

subgenomic

positive-stranded

venezuelan

hp-prrsv

papain-like

noncytopathic

plus-stranded

positive-sense

minus-strand

analysis

3c-like

drug development

autoprotease

medicine

Reaction

the enzymes processes the alphavirus nonstructural polyprotein (nsP1234). The enzyme from Venezuelan equine encephalitis virus shwos a preferens for Gly or Als in position P1', Ala or Cys in P1, and Gly in P2 =

Synonyms

non-structural polyprotein 2, nonstructural polyprotein, nonstructural protein, nonstructural protein 2, ns polyprotein, nsP2, nsP2 protease, nsp2 protein, nsp2pro, p39 nsp2 protease

ECTree

3 Hydrolases

3.4 Acting on peptide bonds (peptidases)

3.4.22 Cysteine endopeptidases

3.4.22.B79 nsP2 protease

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Results	in table
2	Activating Compound
11	AA Sequence
12	Application
13	Cloned(Commentary)
10	Crystallization (Commentary)
9	General Information
18	Inhibitors
23	kcat/KM [mM/s]
35	KM Value [mM]
3	Localization
3	Metals/Ions
2	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
51	Organism
1	pH Optimum
2	pH Range
2	Posttranslational Modification
81	Protein Variants
6	Purification (Commentary)
1	Reaction
45	Reference
1	Storage Stability
37	Substrates and Products (Substrate)
4	Subunits
24	Synonyms
3	Temperature Optimum [°C]
2	Temperature Range [°C]
3	Temperature Stability [°C]
30	Turnover Number [1/s]

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Cloned on EC 3.4.22.B79 - nsP2 protease

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Venezuelan equine encephalitis virus

P27282

752777

DNA coding for nsP2pro (residues Met457-Cys794) amplified and cloned into vector pETBlue1 T7. Expression in Tuner DE3 (pLacI) Escherichia coli cells

Venezuelan equine encephalitis virus

P27282

682878

expressed in BHK-21 cells

Sindbis virus

699901

expressed initially as HisMBP fusion proteins in Escherichia coli BL21(DE3) CodonPlus-RIL cells

3 entries

expression in Escherichia coli

2 entries

expression in Escherichia coli. Proteins are expressed as inclusion bodies under culturing at 37°C, and only partially expressed in a soluble form at 22°C

Chikungunya virus

755412

GFP-encoding DNA fragment cloned into the nsP2 gene by using a transposon-based approach. The entire library of the recombinant nsP2/GFP genes transferred into wild-type Sindbis virus Toto1101 genome. SIN/nsP2GFP/8 and SIN/nsP2GFP/472 expressed in BHK-21 cells

Sindbis virus

681688

GFP-encoding DNA fragment randomly cloned into the nsP2 gene. Entire library of the recombinant nsP2/GFP genes transferred into wild-type SINV Toto1101 genome. BHK-21 cells infected with SIN/nsP2GFP/8 and with both packaged replicons and SIN/nsP2GFP/472 virus

Sindbis virus

699903

Pro39 mutants expressed in Escherichia coli BL21

Semliki forest virus

679819

the gene that encodes a double-mutant IBV nsp2 N-terminal domain (residues 9-393 of the polyprotein, with mutations Q132L and L270F) is expressed in Escherichia coli BL21(DE3) cells

infectious bronchitis virus

706978