Application | Comment | Organism |
---|---|---|
medicine | plays an essential role in virus propagation | Semliki forest virus |
Cloned (Comment) | Organism |
---|---|
Pro39 mutants expressed in Escherichia coli BL21 | Semliki forest virus |
Protein Variants | Comment | Organism |
---|---|---|
A662T | temperature-sensitive in vivo and in vitro | Semliki forest virus |
C478A | inactive | Semliki forest virus |
G577R | temperature-sensitive in vivo and in vitro | Semliki forest virus |
H548A | inactive | Semliki forest virus |
M781T | temperature-sensitive in vivo and in vitro | Semliki forest virus |
N600D | significantly impaired activity. Slower growth rate and smaller bacterial mass as well as a reduced expression level of the corresponding soluble Pro39. Results in preparates with lower degree of purity and shows a tendency to aggregate | Semliki forest virus |
N605D | significantly impaired activity. Slower growth rate and smaller bacterial mass as well as a reduced expression level of the corresponding soluble Pro39. Results in preparates with lower degree of purity and shows a tendency to aggregate | Semliki forest virus |
W549A | inactive | Semliki forest virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
Trx34 | wild-type, at 28°C | Semliki forest virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Semliki forest virus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Pro39 mutants purified by metal affinity chromatography | Semliki forest virus |
Storage Stability | Organism |
---|---|
-70°C, 20 mM HEPES buffer, pH 7.4, 200 mM NaCl, 20% glycerol, 0.1% Tween 20, 1 mM DTT, 1 mM EDTA, 12 months | Semliki forest virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Trx34 + H2O | - |
Semliki forest virus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
nsP2 protease | - |
Semliki forest virus |