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Literature summary for 3.4.22.B79 extracted from

  • Golubtsov, A.; Kaeaeriaeinen, L.; Caldentey, J.
    Characterization of the cysteine protease domain of Semliki Forest virus replicase protein nsP2 by in vitro mutagenesis (2006), FEBS Lett., 580, 1502-1508.
    View publication on PubMed

Application

Application Comment Organism
medicine plays an essential role in virus propagation Semliki forest virus

Cloned(Commentary)

Cloned (Comment) Organism
Pro39 mutants expressed in Escherichia coli BL21 Semliki forest virus

Protein Variants

Protein Variants Comment Organism
A662T temperature-sensitive in vivo and in vitro Semliki forest virus
C478A inactive Semliki forest virus
G577R temperature-sensitive in vivo and in vitro Semliki forest virus
H548A inactive Semliki forest virus
M781T temperature-sensitive in vivo and in vitro Semliki forest virus
N600D significantly impaired activity. Slower growth rate and smaller bacterial mass as well as a reduced expression level of the corresponding soluble Pro39. Results in preparates with lower degree of purity and shows a tendency to aggregate Semliki forest virus
N605D significantly impaired activity. Slower growth rate and smaller bacterial mass as well as a reduced expression level of the corresponding soluble Pro39. Results in preparates with lower degree of purity and shows a tendency to aggregate Semliki forest virus
W549A inactive Semliki forest virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
Trx34 wild-type, at 28°C Semliki forest virus

Organism

Organism UniProt Comment Textmining
Semliki forest virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Pro39 mutants purified by metal affinity chromatography Semliki forest virus

Storage Stability

Storage Stability Organism
-70°C, 20 mM HEPES buffer, pH 7.4, 200 mM NaCl, 20% glycerol, 0.1% Tween 20, 1 mM DTT, 1 mM EDTA, 12 months Semliki forest virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Trx34 + H2O
-
Semliki forest virus ?
-
?

Synonyms

Synonyms Comment Organism
nsP2 protease
-
Semliki forest virus