Any feedback?
Literature summary for 3.4.22.B79 extracted from
- Molecular cloning, overproduction, purification and biochemical characterization of the p39 nsp2 protease domains encoded by three alphaviruses (2009), Protein Expr. Purif., 64, 89-97.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | NaCl has relatively little effect on enzymatic activity up to 200 mM | Semliki forest virus | |
| additional information | NaCl has relatively little effect on enzymatic activity up to 200 mM | Venezuelan equine encephalitis virus |
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | alphavirus nsp2pro proteases are not very useful tools for the removal of affinity tags from recombinant proteins although they do remain promising therapeutic targets for the treatment of a variety of diseases | Sindbis virus |
| drug development | alphavirus nsp2pro proteases are not very useful tools for the removal of affinity tags from recombinant proteins although they do remain promising therapeutic targets for the treatment of a variety of diseases | Semliki forest virus |
| drug development | alphavirus nsp2pro proteases are not very useful tools for the removal of affinity tags from recombinant proteins although they do remain promising therapeutic targets for the treatment of a variety of diseases | Venezuelan equine encephalitis virus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed initially as HisMBP fusion proteins in Escherichia coli BL21(DE3) CodonPlus-RIL cells | Sindbis virus |
| expressed initially as HisMBP fusion proteins in Escherichia coli BL21(DE3) CodonPlus-RIL cells | Semliki forest virus |
| expressed initially as HisMBP fusion proteins in Escherichia coli BL21(DE3) CodonPlus-RIL cells | Venezuelan equine encephalitis virus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K173E | mutation has any influence on the activity of Sindbis virus nsp2pro | Sindbis virus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | inhibition of nsp2pro at or above 2 mM EDTA | Semliki forest virus |  |
| glycerol | activity is reduced by approximately 20fold in the presence of 5% glycerol | Semliki forest virus | |
| glycerol | activity is reduced by approximately 20fold in the presence of 5% glycerol | Venezuelan equine encephalitis virus | |
| additional information | dithiothreitol and tris (2-carboxyethyl) phosphine hydrochloride have no discernable effect on the activity up to 5 mM. The enzyme is able to tolerate up to 10 mM of beta-mercaptoethanol | Semliki forest virus | |
| additional information | nsp2pro can tolerate EDTA up to 10 mM with very little effect. Dithiothreitol and tris (2-carboxyethyl) phosphine hydrochloride have no discernable effect on the activity up to 5 mM. The enzyme is able to tolerate up to 10 mM of beta-mercaptoethanol | Venezuelan equine encephalitis virus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.58 | - |
Semliki forest virus p1/p2 | - |
Venezuelan equine encephalitis virus | |
| 1.2 | - |
Semliki forest virus p1/p2 | - |
Semliki forest virus | |
| 1.27 | - |
Semliki forest virus p3/p4 | - |
Semliki forest virus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Semliki forest virus | - |
- |
- |
| Sindbis virus | - |
- |
- |
| Venezuelan equine encephalitis virus | P27282 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| fusion proteins purified by immobilized metal affinity chromatography, to homogeneity | Sindbis virus |
| fusion proteins purified by immobilized metal affinity chromatography, to homogeneity | Semliki forest virus |
| fusion proteins purified by immobilized metal affinity chromatography, to homogeneity | Venezuelan equine encephalitis virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| MBPNusG-His6 p3/p4 + H2O | - |
Semliki forest virus | ? | - |
? | |
| Semliki forest virus p1/p2 + H2O | - |
Semliki forest virus | ? | - |
? | |
| Semliki forest virus p1/p2 + H2O | - |
Venezuelan equine encephalitis virus | ? | - |
? | |
| Semliki forest virus p3/p4 + H2O | - |
Semliki forest virus | ? | - |
? | |
| thioredoxin fusion protein + H2O | fusion protein is cleaved with greater efficiency by nsp2pro than the original MBP-NusG-His6 substrate with the shorter linker | Semliki forest virus | ? | - |
? | |
| Venezuelan equine encephalitis virus p1/p2 + H2O | - |
Venezuelan equine encephalitis virus | ? | - |
? | |
| Venezuelan equine encephalitis virus p3/p4 + H2O | - |
Venezuelan equine encephalitis virus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nonstructural protein 2 | - |
Sindbis virus |
| nonstructural protein 2 | - |
Semliki forest virus |
| nonstructural protein 2 | - |
Venezuelan equine encephalitis virus |
| nsP2 protease | - |
Venezuelan equine encephalitis virus |
| nsp2pro | - |
Sindbis virus |
| nsp2pro | - |
Semliki forest virus |
| nsp2pro | - |
Venezuelan equine encephalitis virus |
| p39 nsp2 protease | - |
Sindbis virus |
| p39 nsp2 protease | - |
Semliki forest virus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Venezuelan equine encephalitis virus |
| 30 | - |
- |
Semliki forest virus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 30 | - |
Semliki forest virus |
| 4 | 30 | - |
Venezuelan equine encephalitis virus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
activity decreases dramatically at temperatures above 30°C | Semliki forest virus |
| 30 | - |
activity decreases dramatically at temperatures above 30°C | Venezuelan equine encephalitis virus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
Semliki forest virus p1/p2 | - |
Venezuelan equine encephalitis virus | |
| 0.043 | - |
Semliki forest virus p1/p2 | - |
Semliki forest virus | |
| 0.352 | - |
Semliki forest virus p3/p4 | - |
Semliki forest virus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | - |
Venezuelan equine encephalitis virus |
| 6.5 | 8 | - |
Semliki forest virus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
