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3.4.22.B74: SENP6 peptidase

This is an abbreviated version!
For detailed information about SENP6 peptidase, go to the full flat file.

Word Map on EC 3.4.22.B74

Reaction

The enzyme catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO-1, SUMO-2 and SUMO-3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. Cleavage of Gly97-/-His98 bond in the SUMO-1 precursor with release of the propeptide His-Ser-Thr-Val. Cleavage of Gly93-/-Val94 bond in the SUMO-2 precursor with release of the propeptide Val94-Thyr. Cleavage of the Gly92-/-Val93 in the SUMO-3 precursor with release of the propeptide Pro-Glu-Ser-Ser-Leu-Ala-Gly-His-Ser-Phe. The enzyme catalyzes the desumoylation of SUMO-modified promyelocytic leukemia protein. SENP6 prefers the LRGG-/- sequence versus the QTGG-/- sequence. SENP6 is essential for inner kinetochore assembly. =

Synonyms

SENP6, SUSP1

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.B74 SENP6 peptidase

Substrates Products

Substrates Products on EC 3.4.22.B74 - SENP6 peptidase

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(SUMO-1)-beta-galactosidase conjugate + H2O
SUMO-1 + beta-galactosidase
show the reaction diagram
SUSP1 expressed in Escherichia coli cells efficiently releases SUMO-1 from SUMO-1-beta-galactosidase fusion but not from other ubiquitin-like protein fusions, including Smt3-beta-galactosidase
-
-
?
(SUMO-3)-Ran GTPase-activating protein 1 conjugate + H2O
SUMO-3 + Ran GTPase-activating protein 1
show the reaction diagram
SENP6 and SENP7 prefer SUMO2 or SUMO3 in deconjugation reactions with rates comparable with those catalyzed by SENP2. In contrast to SENP2, SENP6 and SENP7 are less able to deconjugate SUMO1-RanGAP1, and products are only detected at enzyme concentrations 100 x higher than that observed for reactions containing SENP2
-
-
?
acetyl-LRGG-7-amido-4-trifluoromethylcoumarin + H2O
acetyl-LRGG + 7-amino-4-trifluoromethylcoumarin
show the reaction diagram
-
SENP6 prefers the LRGG sequence, residues typical of Nedd8 or ubiquitin in the P3 and P4 positions
-
-
?
acetyl-QTGG-7-amido-4-trifluoromethylcoumarin + H2O
acetyl-QTGG + 7-amino-4-trifluoromethylcoumarin
show the reaction diagram
-
very low activity
-
-
?
di-SUMO-2 + H2O
2 SUMO-2
show the reaction diagram
-
-
-
?
di-SUMO-3 + H2O
2 SUMO-3
show the reaction diagram
-
-
-
?
poly-SUMO-2 + H2O
?
show the reaction diagram
-
-
-
?
poly-SUMO-3 + H2O
?
show the reaction diagram
-
-
-
?
SUMO-1 precursor + H2O
SUMO-1 + His-Ser-Thr-Val
show the reaction diagram
SUMO-2 precursor + H2O
SUMO-2 + Val-Tyr
show the reaction diagram
SENP6 exhibits lower rates for processing pre-SUMO-1, pre-SUMO-2, or pre-SUMO3 in comparison with SENP2
-
-
?
SUMO-3 precursor + H2O
SUMO-3 + Val-Pro-Glu-Ser-Ser-Leu-Ala-Gly-His-Ser-Phe
show the reaction diagram
SENP6 exhibits lower rates for processing pre-SUMO-1, pre-SUMO-2, or pre-SUMO-3 in comparison with SENP2
-
-
?
SUMO-modified promyelocytic leukemia protein + H2O
SUMO + promyelocytic leukemia protein
show the reaction diagram
-
-
-
?
SUMOylated TRIM28 + H2O
?
show the reaction diagram
i.e. tripartite motif-containing 28, also called KAP1 or TIF1beta
-
-
?
additional information
?
-