Information on EC 3.4.22.B74 - SENP6 peptidase

Word Map on EC 3.4.22.B74
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Homo sapiens

EC NUMBER
COMMENTARY hide
3.4.22.B74
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
SENP6 peptidase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
The enzyme catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO-1, SUMO-2 and SUMO-3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. Cleavage of Gly97-/-His98 bond in the SUMO-1 precursor with release of the propeptide His-Ser-Thr-Val. Cleavage of Gly93-/-Val94 bond in the SUMO-2 precursor with release of the propeptide Val94-Thyr. Cleavage of the Gly92-/-Val93 in the SUMO-3 precursor with release of the propeptide Pro-Glu-Ser-Ser-Leu-Ala-Gly-His-Ser-Phe. The enzyme catalyzes the desumoylation of SUMO-modified promyelocytic leukemia protein. SENP6 prefers the LRGG-/- sequence versus the QTGG-/- sequence. SENP6 is essential for inner kinetochore assembly.
show the reaction diagram
-
-
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
-
the enzyme belongs to the SENP/ULP protease family
malfunction
physiological function
-
SENP6 is essential for inner kinetochore assembly. Balanced activities of SENP6 and RNF4 control vertebrate kinetochore assembly through SUMO-targeted destabilization of inner plate components
additional information
-
active site loop1 insertion is the determinant for the SUMO2/3 activity and specificity of SENP6, role of the SENP6/7-loop1 insertion in chain dismantling
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(SUMO-1)-beta-galactosidase conjugate + H2O
SUMO-1 + beta-galactosidase
show the reaction diagram
SUSP1 expressed in Escherichia coli cells efficiently releases SUMO-1 from SUMO-1-beta-galactosidase fusion but not from other ubiquitin-like protein fusions, including Smt3-beta-galactosidase
-
-
?
(SUMO-3)-Ran GTPase-activating protein 1 conjugate + H2O
SUMO-3 + Ran GTPase-activating protein 1
show the reaction diagram
-
SENP6 and SENP7 prefer SUMO2 or SUMO3 in deconjugation reactions with rates comparable with those catalyzed by SENP2. In contrast to SENP2, SENP6 and SENP7 are less able to deconjugate SUMO1-RanGAP1, and products are only detected at enzyme concentrations 100 x higher than that observed for reactions containing SENP2
-
-
?
acetyl-LRGG-7-amido-4-trifluoromethylcoumarin + H2O
acetyl-LRGG + 7-amino-4-trifluoromethylcoumarin
show the reaction diagram
-
SENP6 prefers the LRGG sequence, residues typical of Nedd8 or ubiquitin in the P3 and P4 positions
-
-
?
acetyl-QTGG-7-amido-4-trifluoromethylcoumarin + H2O
acetyl-QTGG + 7-amino-4-trifluoromethylcoumarin
show the reaction diagram
-
very low activity
-
-
?
di-SUMO-2 + H2O
2 SUMO-2
show the reaction diagram
-
-
-
-
?
di-SUMO-3 + H2O
2 SUMO-3
show the reaction diagram
-
-
-
-
?
poly-SUMO-2 + H2O
?
show the reaction diagram
-
-
-
-
?
poly-SUMO-3 + H2O
?
show the reaction diagram
-
-
-
-
?
SUMO-1 precursor + H2O
SUMO-1 + His-Ser-Thr-Val
show the reaction diagram
SUMO-2 precursor + H2O
SUMO-2 + Val-Tyr
show the reaction diagram
-
SENP6 exhibits lower rates for processing pre-SUMO-1, pre-SUMO-2, or pre-SUMO3 in comparison with SENP2
-
-
?
SUMO-3 precursor + H2O
SUMO-3 + Val-Pro-Glu-Ser-Ser-Leu-Ala-Gly-His-Ser-Phe
show the reaction diagram
-
SENP6 exhibits lower rates for processing pre-SUMO-1, pre-SUMO-2, or pre-SUMO-3 in comparison with SENP2
-
-
?
SUMO-modified promyelocytic leukemia protein + H2O
SUMO + promyelocytic leukemia protein
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
SUMO-modified promyelocytic leukemia protein + H2O
SUMO + promyelocytic leukemia protein
show the reaction diagram
-
-
-
-
?
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
truncated SUMO-2
-
whereas truncated SUMO-2 and -3 can substantially enhance SENP6 activity, neither truncated SUMO-1 nor truncated Nedd8 nor ubiquitin has this ability
-
truncated SUMO-3
-
whereas truncated SUMO-2 and -3 can substantially enhance SENP6 activity, neither truncated SUMO-1 nor truncated Nedd8 nor ubiquitin has this ability
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.8
-
assay at
8
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23
-
assay at
37
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
little activity
Manually annotated by BRENDA team
-
reproductive organs, such as testis, ovary, and prostate, contain much higher amounts of SUSP1 mRNA than colon and peripheral blood leukocyte
Manually annotated by BRENDA team
-
reproductive organs, such as testis, ovary, and prostate, contain much higher amounts of SUSP1 mRNA than colon and peripheral blood leukocyte
Manually annotated by BRENDA team
-
reproductive organs, such as testis, ovary, and prostate, contain much higher amounts of SUSP1 mRNA than colon and peripheral blood leukocyte
Manually annotated by BRENDA team
-
reproductive organs, such as testis, ovary, and prostate, contain much higher amounts of SUSP1 mRNA than colon and peripheral blood leukocyte
Manually annotated by BRENDA team
-
reproductive organs, such as testis, ovary, and prostate, contain much higher amounts of SUSP1 mRNA than colon and peripheral blood leukocyte
Manually annotated by BRENDA team
additional information
-
no or little SUSP1 mRNA in liver, lung, kidney, pancreas, spleen, thymus, heart, and skeletal muscle
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
SUSP1 is exclusively localized to the cytoplasm of NIH3T3 and HeLa cells
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
126116
-
x * 126116, calculated from sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 126116, calculated from sequence
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-