3.4.22.70: sortase A

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For detailed information about sortase A, go to the full flat file.

Reaction

The enzyme catalyses a cell wall sorting reaction in which a surface protein with a sorting signal containing a LPXTG motif is cleaved between the Thr and Gly residue. The resulting threonine carboxyl end of the protein is covalently attached to a pentaglycine cross-bridge of peptidoglycan. =

Synonyms

C60.001, sortase A, sortase A transpeptidase, sortase SrtA, sortase transpeptidase, SrtA, SrtA protein, SrtA sortase

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.22 Cysteine endopeptidases

                3.4.22.70 sortase A

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Results	in table
388	AA Sequence
23	Application
1	CAS Registry Number
30	Cloned(Commentary)
4	Crystallization (Commentary)
25	General Information
166	IC50 Value
214	Inhibitors
4	kcat/KM [mM/s]
4	Ki Value [mM]
43	KM Value [mM]
5	Localization
7	Metals/Ions
10	Molecular Weight [Da]
18	Natural Substrates/ Products (Substrates)
127	Organism
17	PDB ID
5	pH Optimum
2	pH Range
56	Protein Variants
30	Purification (Commentary)
1	Reaction
1	Reaction Type
93	Reference
1	Source Tissue
1	Specific Activity [micromol/min/mg]
108	Substrates and Products (Substrate)
9	Subunits
65	Synonyms
4	Temperature Optimum [°C]
32	Turnover Number [1/s]

Crystallization

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Crystallization on EC 3.4.22.70 - sortase A

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

crystals are grown by the hanging-drop technique with a protein concentration of 50 mg/ml in 25 mM MES buffer, pH 6.35. The crystallization conditions include 3.2 M ammonium sulfate, 0.1 M NaCl, and trace amounts of ethylene glycol. Crystal structure of native SrtA, of an active-site mutant of SrtA, and of the mutant SrtA complexed with its substrate LPETG peptide

Staphylococcus aureus

-

647385

wild-type enzyme SrtA apo and mutant enzyme C184A bound to LPETG peptide sequence, crystal structure analysis

Staphylococcus aureus

Q95446

732881

-

Streptococcus agalactiae

Q8DZY1

718286

hanging drop method of vapor diffusion at 20°C, SrtA residues Val82-Thr249 (catalytic domain)

Streptococcus pyogenes

Q99ZN4

693252