3.4.22.70: sortase A
This is an abbreviated version!
For detailed information about sortase A, go to the full flat file.
Word Map on EC 3.4.22.70
-
3.4.22.70
-
aureus
-
staphylococcus
-
lpxtg
-
streptococcus
-
peptidoglycan
-
transpeptidation
-
sortase-mediated
-
a-mediated
-
adhesins
-
bioconjugation
-
antivirulence
-
mutans
-
anti-infective
-
pilins
-
transpeptidases
-
oligoglycine
-
cross-bridges
-
pentaglycine
-
wall-anchored
-
molecular biology
-
azide-alkyne
-
analysis
-
biotechnology
-
drug development
-
synthesis
-
medicine
- 3.4.22.70
- aureus
- staphylococcus
-
lpxtg
- streptococcus
- peptidoglycan
-
transpeptidation
-
sortase-mediated
-
a-mediated
- adhesins
-
bioconjugation
-
antivirulence
- mutans
-
anti-infective
- pilins
- transpeptidases
-
oligoglycine
-
cross-bridges
- pentaglycine
-
wall-anchored
- molecular biology
-
azide-alkyne
- analysis
- biotechnology
- drug development
- synthesis
- medicine
Reaction
The enzyme catalyses a cell wall sorting reaction in which a surface protein with a sorting signal containing a LPXTG motif is cleaved between the Thr and Gly residue. The resulting threonine carboxyl end of the protein is covalently attached to a pentaglycine cross-bridge of peptidoglycan. =
Synonyms
C60.001, sortase A, sortase A transpeptidase, sortase SrtA, sortase transpeptidase, SrtA, SrtA protein, SrtA sortase
ECTree
Advanced search results
Purification
Purification on EC 3.4.22.70 - sortase A
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
His-Trap column chromatography
HiTrap Chelating HP column chromatography and Superdex 75 gel filtration
-
N-terminally His6-tagged SrtADELTAN24 expressed in Escherichia coli BL21
-
recombinant enzyme, SrtA residues Val82-Thr249 (catalytic domain)
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromaatography and dialysis
-
recombinant His6-tagged enzyme catalytic domain from Escherichia coli strain BL21(DE3) by immobilized metal affinity chromatograph
-
recombinant mutant and modified enzymes from Escherichia coli by cation and anion exchange chromatography
-
recombinant nontagged truncated enzyme mutant variant SrtADELTA59 from Escherichia coli strain BL21(DE3) by cation and anion exchange chromatography, dialysis, and gel filtration, recombinant His6-tagged truncated enzyme mutant variant SrtADELTA59 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
-
Talon His-affinity resin column chromatography and Sephacryl-100 gel filtration
-