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3.4.22.70: sortase A

This is an abbreviated version!
For detailed information about sortase A, go to the full flat file.

Word Map on EC 3.4.22.70

Reaction

The enzyme catalyses a cell wall sorting reaction in which a surface protein with a sorting signal containing a LPXTG motif is cleaved between the Thr and Gly residue. The resulting threonine carboxyl end of the protein is covalently attached to a pentaglycine cross-bridge of peptidoglycan. =

Synonyms

C60.001, sortase A, sortase A transpeptidase, sortase SrtA, sortase transpeptidase, SrtA, SrtA protein, SrtA sortase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.70 sortase A

Purification

Purification on EC 3.4.22.70 - sortase A

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by Ni-NTA affinity chromatography
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His-Trap column chromatography
HisTrapHP column chromatography, gel filtration
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HiTrap Chelating HP column chromatography and Superdex 75 gel filtration
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N-terminally His6-tagged SrtADELTAN24 expressed in Escherichia coli BL21
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Ni-NTA column chromatography
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Ni2+-HisTrap column chromatography
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of SrtADELTAN59 and mutant proteins
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purification of SrtADELTAN59 and SrtA wild-type proteins
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recombinant enzyme, SrtA residues Val82-Thr249 (catalytic domain)
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromaatography and dialysis
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recombinant His6-tagged enzyme catalytic domain from Escherichia coli strain BL21(DE3) by immobilized metal affinity chromatograph
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recombinant mutant and modified enzymes from Escherichia coli by cation and anion exchange chromatography
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recombinant nontagged truncated enzyme mutant variant SrtADELTA59 from Escherichia coli strain BL21(DE3) by cation and anion exchange chromatography, dialysis, and gel filtration, recombinant His6-tagged truncated enzyme mutant variant SrtADELTA59 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
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recombinant sortase A-GST fusion protein
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recombinant SrtA lacking the amino-terminal 24 amino acids
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recombinant SrtADELTAN24 in a His6-tagged form
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Talon His-affinity resin column chromatography and Sephacryl-100 gel filtration
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Talon resin column chromatography
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