3.4.22.70: sortase A
This is an abbreviated version!
For detailed information about sortase A, go to the full flat file.
Word Map on EC 3.4.22.70
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3.4.22.70
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aureus
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staphylococcus
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lpxtg
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streptococcus
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peptidoglycan
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transpeptidation
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sortase-mediated
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a-mediated
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adhesins
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bioconjugation
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antivirulence
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mutans
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anti-infective
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pilins
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transpeptidases
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oligoglycine
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cross-bridges
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pentaglycine
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wall-anchored
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molecular biology
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azide-alkyne
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analysis
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biotechnology
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drug development
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synthesis
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medicine
- 3.4.22.70
- aureus
- staphylococcus
-
lpxtg
- streptococcus
- peptidoglycan
-
transpeptidation
-
sortase-mediated
-
a-mediated
- adhesins
-
bioconjugation
-
antivirulence
- mutans
-
anti-infective
- pilins
- transpeptidases
-
oligoglycine
-
cross-bridges
- pentaglycine
-
wall-anchored
- molecular biology
-
azide-alkyne
- analysis
- biotechnology
- drug development
- synthesis
- medicine
Reaction
The enzyme catalyses a cell wall sorting reaction in which a surface protein with a sorting signal containing a LPXTG motif is cleaved between the Thr and Gly residue. The resulting threonine carboxyl end of the protein is covalently attached to a pentaglycine cross-bridge of peptidoglycan. =
Synonyms
C60.001, sortase A, sortase A transpeptidase, sortase SrtA, sortase transpeptidase, SrtA, SrtA protein, SrtA sortase
ECTree
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Cloned
Cloned on EC 3.4.22.70 - sortase A
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DNA sequence encoding SrtA residues Val82-Thr249, expression in Escherichia coli BL21
expressed in Escherichia coli BL21(DE3) cells
gene srtA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree
gene srtA, recombinant expression of His6-tagged enzyme catalytic domain, comprising residues 60-206, in Escherichia coli strain BL21(DE3)
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N-terminally His6-tagged SrtA lacking the amino-terminal 24 amino acids is expressed in BL21(DE3) cells containing the plasmid pET15bSrtADN24
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N-terminally His6-tagged SrtADELTAN24 is expressed in Escherichia coli BL21
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recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
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recombinant expression of His6-tagged and of nontagged truncated enzyme mutant variant SrtADELTA59 in Escherichia coli strain BL21(DE3)
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recombinant expression of linear truncated enzyme mutant LsrtADELTA59 in Escherichia coli strain BL21(DE3), development of an expression system in Escherichia coli that allows production of circularly closed sortase A truncated mutant CsrtADELTA59, overview. Efficient enzyme cyclization is achieved by Synechocystis sp. PCC6803 intein-mediated post-translational splicing, NMR spectroscopy of 15N-labeled LsrtADELTAN59 and CsrtADELTAN59
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recombinant mature sortase A (without the N-terminal signal peptide) is produced as GST fusion in Escherichia coli
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