Literature summary for 3.4.22.70 extracted from

Race, P.R.; Bentley, M.L.; Melvin, J.A.; Crow, A.; Hughes, R.K.; Smith, W.D.; Sessions, R.B.; Kehoe, M.A.; McCafferty, D.G.; Banfield, M.J.
Crystal structure of Streptococcus pyogenes sortase A: implications for sortase mechanism (2009), J. Biol. Chem., 284, 6924-6933.

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Cloned(Commentary)

Cloned (Comment)	Organism
DNA sequence encoding SrtA residues Val82-Thr249, expression in Escherichia coli BL21	Streptococcus pyogenes

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop method of vapor diffusion at 20°C, SrtA residues Val82-Thr249 (catalytic domain)	Streptococcus pyogenes

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pyogenes	Q99ZN4	-	-
Streptococcus pyogenes SF370	Q99ZN4	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme, SrtA residues Val82-Thr249 (catalytic domain)	Streptococcus pyogenes

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Abz-LPETGG-Dap(Dnp)-NH2 + Ala-Ala	transpeptidation of LPXTG-containing peptides to the cell-wall precursor mimic AlaAla	Streptococcus pyogenes	Abz-LPETAA + GG-Dap(Dnp)-NH2	-	?
Abz-LPETGG-Dap(Dnp)-NH2 + Ala-Ala	transpeptidation of LPXTG-containing peptides to the cell-wall precursor mimic AlaAla	Streptococcus pyogenes SF370	Abz-LPETAA + GG-Dap(Dnp)-NH2	-	?

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Release 2023.1 (January 2023)