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3.4.22.70: sortase A

This is an abbreviated version!
For detailed information about sortase A, go to the full flat file.

Word Map on EC 3.4.22.70

Reaction

The enzyme catalyses a cell wall sorting reaction in which a surface protein with a sorting signal containing a LPXTG motif is cleaved between the Thr and Gly residue. The resulting threonine carboxyl end of the protein is covalently attached to a pentaglycine cross-bridge of peptidoglycan. =

Synonyms

C60.001, sortase A, sortase A transpeptidase, sortase SrtA, sortase transpeptidase, SrtA, SrtA protein, SrtA sortase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.70 sortase A

Substrates Products

Substrates Products on EC 3.4.22.70 - sortase A

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-aminobenzoyl-LPATG-diaminopropionic acid + H2O
2-aminobenzoyl-LPAT + G-diaminopropionic acid
show the reaction diagram
-
efficient cleavage
-
-
?
2-aminobenzoyl-LPNTA-diaminopropionic acid + H2O
2-aminobenzoyl-LPNT + A-diaminopropionic acid
show the reaction diagram
-
small amount of cleavage
-
-
?
4-([4-(dimethylamino)phenyl]-azo)-benzoyl-QALPETGEE-((2-aminoethyl)-amino)naphthalene-1-sulfonic acid + H2O
?
show the reaction diagram
-
a catalytically important and conserved binding surface is formed by residues A118, T180 and I182. R197 is also required for catalysis
-
-
?
4-[[4'-(dimethylamino)phenyl]azo]-benzoyl-Gln-Ala-Leu-Pro-Glu-Thr-Gly-Glu-Glu-5-[(2'-aminoethyl)-amino]naphthalenesulfonic acid + H2O
4-[[4'-(dimethylamino)phenyl]azo]-benzoyl-Gln-Ala-Leu-Pro-Glu-Thr + Gly-Glu-Glu-5-[(2'-aminoethyl)-amino]naphthalenesulfonic acid
show the reaction diagram
-
-
-
-
?
4-[[4'-(dimethylamino)phenyl]azo]-benzoyl-Leu-Pro-Glu-Thr-Gly-5-[(2'-aminoethyl)-amino]naphthalenesulfonic acid + H2O
4-[[4'-(dimethylamino)phenyl]azo]-benzoyl-Leu-Pro-Glu-Thr + Gly-5-[(2'-aminoethyl)-amino]naphthalenesulfonic acid
show the reaction diagram
-
-
-
-
?
5(6)-carboxyfluorescein-6-aminohexanoic acid-LPKTGGRR-NH2 + H2O
?
show the reaction diagram
-
-
-
-
?
A33-LPETG-His6 + H2O
?
show the reaction diagram
-
A33 antigen extracellular domain bearing a His6 tag
-
-
?
Abz-KVENPQTNAGT-Dap(Dnp)-NH2 + GGGGG
Abz-KVENPQTGGGGG + Gly-Dap(DNP)-NH2
show the reaction diagram
-
-
-
-
?
Abz-LPETG-Dap(Dnp) + GGGGG
Abz-LPETGGGGG + G-Dap(Dnp)
show the reaction diagram
-
-
-
-
?
Abz-LPETG-Dap(Dnp) + Gly5
?
show the reaction diagram
-
-
-
-
?
Abz-LPETG-Dap(Dnp) + H2O
?
show the reaction diagram
Abz-LPETG-Dap(Dnp) + H2O
Abz-LPET + G-Dap(Dnp)
show the reaction diagram
Abz-LPETG-Dap(Dnp)-NH2 + GGGGG
?
show the reaction diagram
-
-
-
-
?
Abz-LPETG-Dap(Dnp)-NH2 + GGGGG
Abz-LPETGGGGGG + Gly-Dap(Dnp)-NH2
show the reaction diagram
-
-
-
-
?
Abz-LPETG-Dap(Dnp)-NH2 + Gly5
?
show the reaction diagram
-
transpeptidation of LPXTG-containing peptides to the cell-wall precursor mimic NH2-Ala2
-
-
?
Abz-LPETG-Dap(Dnp)-NH2 + Gly5
Abz-LPETGGGGG-OH + Gly-Dap(Dnp)-NH2
show the reaction diagram
-
-
-
-
?
Abz-LPETG-Dap(Dnp)-NH2 + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-LPETGG-Dap(Dnp)-NH2 + Ala-Ala
Abz-LPETAA + GG-Dap(Dnp)-NH2
show the reaction diagram
Abz-LPETGG-Dap(Dnp)-NH2 + GGGGG
Abz-LPETGGGGGG + Gly-Dap(Dnp)-NH2
show the reaction diagram
-
-
-
-
?
Abz-LPKTGK(Dnp)KK + GGGWW
Abz-LPKTGGGWW + GK(Dnp)KK
show the reaction diagram
-
substrate peptide 1 (Abz-LPKTGK(Dnp)KK) and acceptor peptide 2 (GGGWW)
-
-
?
acetyl-ooocctcttacctcagttacaoooLPKTGGR-NH2 + H-GGGKLALKLALKALKAALKLA-NH2
acetyl-ooocctcttacctcagttacaoooLPKTGGGKLALKLALKALKAALKLA-NH2 + H-GGR-NH2
show the reaction diagram
-
-
-
-
?
agmatine + YALPETGK
(NH2)2-CN-(CH2)4-NH-(CO)-TEPLAY + ?
show the reaction diagram
-
transpeptidase reaction. The initially formed acyl-enzyme intermediate undergoes a hydrolysis followed by intra-molecular transpeptidation
-
-
?
AHLPKTGLR + 5-aminopentan-1-ol
?
show the reaction diagram
-
-
-
-
?
AHLPKTGLR + 6-aminohexyl 4-O-beta-D-galactopyranosyl-beta-D-glucopyranoside
?
show the reaction diagram
-
-
-
-
?
AHLPKTGLR + N-(2-(2-ethoxyethoxy)ethanamine)biotin amide
?
show the reaction diagram
-
-
-
-
?
AHLPKTGLR + N-hexylbiotin amide
?
show the reaction diagram
-
-
-
-
?
AHLPKTGLR-NH2 + triglycine
?
show the reaction diagram
-
-
-
-
?
aminobenzoyl-LPETG-dinitrophenyl ester + Gly-Gly-Gly
aminobenzoyl-LPETGGG + Gly-dinitrophenyl ester
show the reaction diagram
-
HPLC results provide direct evidence for the formation of the kinetically competent acyl enzyme intermediate
-
-
?
Dabcyl-LPETG-EDANS + H2O
Dabcyl-LPET + Gly-EDANS
show the reaction diagram
-
-
-
-
?
Dabcyl-QALPETGEE-Edans + H2O
Dabcyl-QALPET + GEE-Edans
show the reaction diagram
Dabcyl-SFLPKTGM-EDANS + H2O
?
show the reaction diagram
Dnp-AQALPETGEE-NH2 + Gly5
?
show the reaction diagram
-
-
-
-
?
Dns-LPKTGGRR + GGGWW
Dns-LPKTGGGWW + GGRR
show the reaction diagram
-
dansyl-labeled Dns-LPKTGGRR substrate peptide and acceptor peptide 2 (GGGWW)
-
-
?
GST-LPETG + G-eGFP
?
show the reaction diagram
o-aminobenzoyl-Leu-Pro-Glu-Thr-Gly-2,4-dinitrophenyl ester + Gly-Gly-Gly
o-aminobenzoyl-Leu-Pro-Glu-Thr-Gly-Gly-Gly + Gly-2,4-dinitrophenyl ester
show the reaction diagram
-
ping-pong mechanism in which a common acyl-enzyme intermediate is formed in transpeptidation and hydrolysis. The nucleophile binding site of the enzyme is specific for diglycine. The S1' and S2' sites of the sortase both prefer a glycine residue, the S1' site is exclusively selective for glycine
-
-
?
o-aminobenzoyl-Leu-Pro-Glu-Thr-Gly-2,4-dinitrophenyl ester + H2O
o-aminobenzoyl-Leu-Pro-Glu-Thr + Gly-2,4-dinitrophenyl ester
show the reaction diagram
-
ping-pong mechanism in which a common acyl-enzyme intermediate is formed in transpeptidation and hydrolysis. The nucleophile binding site of the enzyme is specific for diglycine. The S1' and S2' sites of the sortase both prefer a glycine residue, the S1' site is exclusively selective for glycine
-
-
?
o-aminobenzoyl-LPETG-2,4-dinitrophenyl + H2O
o-aminobenzoyl-LPETG + 2,4-dinitrophenol
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-LPETG-2,4-dinitrophenyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
PrtP protein + H2O
?
show the reaction diagram
recombinant Helicobacter pylori R1,3-fucosyltransferase + triglycine
?
show the reaction diagram
-
-
-
-
?
recombinant human beta1,4-galactosyltransferase + N-(2-(2-ethoxyethoxy)ethanamine)biotin amide
?
show the reaction diagram
-
-
-
-
?
recombinant human beta1,4-galactosyltransferase + N-hexylbiotin amide
?
show the reaction diagram
-
-
-
-
?
YALPETGK + H2O
YALPET + Gly-Lys
show the reaction diagram
-
-
-
-
?
YbeF protein + H2O
?
show the reaction diagram
YhgE protein + H2O
?
show the reaction diagram
YndF protein + H2O
?
show the reaction diagram
YwfG protein + H2O
?
show the reaction diagram
[4-(4-dimethylaminophenylazo)benzoic acid]-QALPETGEE-[5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid] + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-