3.4.22.70: sortase A
This is an abbreviated version!
For detailed information about sortase A, go to the full flat file.
Word Map on EC 3.4.22.70
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3.4.22.70
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aureus
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staphylococcus
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lpxtg
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streptococcus
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peptidoglycan
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transpeptidation
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sortase-mediated
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a-mediated
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adhesins
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bioconjugation
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antivirulence
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mutans
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anti-infective
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pilins
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transpeptidases
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oligoglycine
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cross-bridges
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pentaglycine
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wall-anchored
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molecular biology
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azide-alkyne
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analysis
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biotechnology
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drug development
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synthesis
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medicine
- 3.4.22.70
- aureus
- staphylococcus
-
lpxtg
- streptococcus
- peptidoglycan
-
transpeptidation
-
sortase-mediated
-
a-mediated
- adhesins
-
bioconjugation
-
antivirulence
- mutans
-
anti-infective
- pilins
- transpeptidases
-
oligoglycine
-
cross-bridges
- pentaglycine
-
wall-anchored
- molecular biology
-
azide-alkyne
- analysis
- biotechnology
- drug development
- synthesis
- medicine
Reaction
The enzyme catalyses a cell wall sorting reaction in which a surface protein with a sorting signal containing a LPXTG motif is cleaved between the Thr and Gly residue. The resulting threonine carboxyl end of the protein is covalently attached to a pentaglycine cross-bridge of peptidoglycan. =
Synonyms
C60.001, sortase A, sortase A transpeptidase, sortase SrtA, sortase transpeptidase, SrtA, SrtA protein, SrtA sortase
ECTree
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KM Value
KM Value on EC 3.4.22.70 - sortase A
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1.18
agmatine
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at 37°C, pH not specified in the publication, transpeptidation reaction
0.79 - 11.2
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide
4.69
-
37°C, pH 7.5, mutant enzyme R197A
6.56
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2
-
37°C, pH 7.5, mutant enzyme V168A
6.74
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2
-
37°C, pH 7.5, mutant enzyme E171A
8.13
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2
-
37°C, pH 7.5, mutant enzyme D170A
8.7
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2
-
37°C, pH 7.5, mutant enzyme G167A
8.76
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2
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37°C, pH 7.5, wild-type enzyme
9.14
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2
-
37°C, pH 7.5, mutant enzyme L169A
10.4
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2
-
37°C, pH 7.5, mutant enzyme R197K
12.7
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2
-
37°C, pH 7.5, mutant enzyme Q172A
13.7
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2
-
37°C, pH 7.5, mutant enzyme R197Cit
0.013
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pH 7.5, 37°C, recombinant linear truncated mutant LSrtADELTAN59 enzyme
0.014
Dabcyl-QALPETGEE-Edans
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pH 7.5, 37°C, recombinant circular truncated mutant CSrtADELTAN59 enzyme
0.041
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pH 7.5, transpeptidation with o-aminobenzoyl-Leu-Pro-Glu-Thr-Gly-2,4-dinitrophenol
0.79
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide
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mutant enzyme
3.8
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide
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mutant enzyme
4.7
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide
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mutant enzyme
6.7
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide
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mutant enzyme
8.5
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide
-
mutant enzyme
10.6
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide
-
wild-type enzyme
11.2
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide
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mutant enzyme
0.02
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hydrolysis reaction
0.117
o-aminobenzoyl-Leu-Pro-Glu-Thr-Gly-2,4-dinitrophenyl ester
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pH 7.5, transpeptidation with Gly-Gly-Gly
0.035
-
sortase A DELTA64 (residues Asp65Lys210), in 20 mM HEPES, pH 7.5
0.038
o-aminobenzoyl-LPETG-2,4-dinitrophenyl
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sortase A DELTA56 (residues Asp-57-Lys210), in 20 mM HEPES, pH 7.5
0.00013
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mutant enzyme E108A
0.0018
o-aminobenzoyl-LPETG-2,4-dinitrophenyl ester
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mutant enzyme E171A
0.0087
o-aminobenzoyl-LPETG-2,4-dinitrophenyl ester
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mutant enzyme D170A