3.4.21.66: Thermitase

This is an abbreviated version!
For detailed information about Thermitase, go to the full flat file.

Word Map on EC 3.4.21.66

3.4.21.66

subtilisins

3.4.21.14

carlsberg

subtilisin-like

subtilases

subtilisin-type

eglin-c

biotechnology

analysis

Reaction

Hydrolysis of proteins, including collagen =

Synonyms

Alkaline protease, EC 3.4.21.14, EC 3.4.4.16, PLLA-degrading enzyme, PrlA, Proteinase, Thermoactinomyces vulgaris serine, proteolysin, Thermoactinomyces vulgaris serine proteinase, Thermophilic Streptomyces serine proteinase, thermostable serine protease

ECTree

3 Hydrolases

3.4 Acting on peptide bonds (peptidases)

3.4.21 Serine endopeptidases

3.4.21.66 Thermitase

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Results	in table
7	Activating Compound
131	AA Sequence
2	Application
1	CAS Registry Number
3	Cloned(Commentary)
10	Crystallization (Commentary)
5	General Information
6	General Stability
56	Inhibitors
6	kcat/KM [mM/s]
7	KM Value [mM]
7	Localization
6	Metals/Ions
14	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
47	Organism
4	PDB ID
13	pH Optimum
2	pH Range
4	pH Stability
1	Posttranslational Modification
4	Protein Variants
11	Purification (Commentary)
1	Reaction
1	Reaction Type
47	Reference
6	Source Tissue
6	Specific Activity [micromol/min/mg]
47	Substrates and Products (Substrate)
5	Subunits
14	Synonyms
14	Temperature Optimum [°C]
2	Temperature Range [°C]
10	Temperature Stability [°C]
6	Turnover Number [1/s]

Temperature Optimum

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Temperature Optimum on EC 3.4.21.66 - Thermitase

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40 - 70

Coprothermobacter proteolyticus

B5Y6G0

substrate N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide

731179

Anoxybacillus sp. KP1

753214

4 entries

Nocardiopsis alba

immobilized enzyme on chitosan, silica gel 100-200, alginate and glutaraldehyde activated chitosan and nitrocellulose membrane

753026

Thermoactinomyces vulgaris

amino acid 4-nitroanilides

29565

Nocardiopsis alba

immobilized enzyme on amberlite IR 120, DEAE Sephadex A50, and polyacrylamide

753026

2 entries

Thermoactinomyces vulgaris

casein

29565

additional information

Thermoactinomyces vulgaris

the temperature optimum increases with incresing MW of substrate

29563