3.4.21.66: Thermitase

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For detailed information about Thermitase, go to the full flat file.

Word Map on EC 3.4.21.66

3.4.21.66

subtilisins

3.4.21.14

carlsberg

subtilisin-like

subtilases

subtilisin-type

eglin-c

biotechnology

analysis

Reaction

Hydrolysis of proteins, including collagen =

Synonyms

Alkaline protease, EC 3.4.21.14, EC 3.4.4.16, PLLA-degrading enzyme, PrlA, Proteinase, Thermoactinomyces vulgaris serine, proteolysin, Thermoactinomyces vulgaris serine proteinase, Thermophilic Streptomyces serine proteinase, thermostable serine protease

ECTree

3 Hydrolases

3.4 Acting on peptide bonds (peptidases)

3.4.21 Serine endopeptidases

3.4.21.66 Thermitase

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Results	in table
7	Activating Compound
131	AA Sequence
2	Application
1	CAS Registry Number
3	Cloned(Commentary)
10	Crystallization (Commentary)
5	General Information
6	General Stability
56	Inhibitors
6	kcat/KM [mM/s]
7	KM Value [mM]
7	Localization
6	Metals/Ions
14	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
47	Organism
4	PDB ID
13	pH Optimum
2	pH Range
4	pH Stability
1	Posttranslational Modification
4	Protein Variants
11	Purification (Commentary)
1	Reaction
1	Reaction Type
47	Reference
6	Source Tissue
6	Specific Activity [micromol/min/mg]
47	Substrates and Products (Substrate)
5	Subunits
14	Synonyms
14	Temperature Optimum [°C]
2	Temperature Range [°C]
10	Temperature Stability [°C]
6	Turnover Number [1/s]

Molecular Weight

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Molecular Weight on EC 3.4.21.66 - Thermitase

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11000

Thermoactinomyces vulgaris

Thermoactinomyces vulgaris, gel filtration

29566

21500

Streptomyces rectus var. proteolyticus

Streptomyces rectus var. proteolyticus, gel filtration, sedimentation equilibrium

29534

26200

Streptomyces rectus var. proteolyticus

Streptomyces rectus var. proteolyticus, sedimentation diffusion measurement

29535

28000

3 entries

28369

Thermoactinomyces vulgaris

1 * 28369, Thermoactinomyces vulgaris, calculation from primary structure

29533

28370

Thermoactinomyces vulgaris

Thermoactinomyces vulgaris, calculation from primary structure

29534

29100

Streptomyces rectus var. proteolyticus

Streptomyces rectus var. proteolyticus, amino acid analysis

29535

37400

Thermoactinomyces vulgaris

29550

8500

Laceyella sacchari

recombinant enzyme, gel filtration, thermitase interactions with the column matrix can delay elution of the protein and lead to underestimation of the molecular size

732871

additional information

3 entries