3.4.21.66: Thermitase

This is an abbreviated version!
For detailed information about Thermitase, go to the full flat file.

Word Map on EC 3.4.21.66

3.4.21.66

subtilisins

3.4.21.14

carlsberg

subtilisin-like

subtilases

subtilisin-type

eglin-c

biotechnology

analysis

Reaction

Hydrolysis of proteins, including collagen =

Synonyms

Alkaline protease, EC 3.4.21.14, EC 3.4.4.16, PLLA-degrading enzyme, PrlA, Proteinase, Thermoactinomyces vulgaris serine, proteolysin, Thermoactinomyces vulgaris serine proteinase, Thermophilic Streptomyces serine proteinase, thermostable serine protease

ECTree

3 Hydrolases

3.4 Acting on peptide bonds (peptidases)

3.4.21 Serine endopeptidases

3.4.21.66 Thermitase

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Results	in table
7	Activating Compound
131	AA Sequence
2	Application
1	CAS Registry Number
3	Cloned(Commentary)
10	Crystallization (Commentary)
5	General Information
6	General Stability
56	Inhibitors
6	kcat/KM [mM/s]
7	KM Value [mM]
7	Localization
6	Metals/Ions
14	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
47	Organism
4	PDB ID
13	pH Optimum
2	pH Range
4	pH Stability
1	Posttranslational Modification
4	Protein Variants
11	Purification (Commentary)
1	Reaction
1	Reaction Type
47	Reference
6	Source Tissue
6	Specific Activity [micromol/min/mg]
47	Substrates and Products (Substrate)
5	Subunits
14	Synonyms
14	Temperature Optimum [°C]
2	Temperature Range [°C]
10	Temperature Stability [°C]
6	Turnover Number [1/s]

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Engineering on EC 3.4.21.66 - Thermitase

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Go to Protein Variants Search

C182A/C201A

Coprothermobacter proteolyticus

B5Y6G0

site-directed mutagenesis, elimination of the disulfide bond, the mutant shows increased activity but reduced thermostability compared to the wild-type enzyme

731179

yes

2 entries

additional information

Thermoactinomyces vulgaris

P04072

computational procedure OptGraft for placing a novel binding pocket onto a protein structure so as its geometry is minimally perturbed. Transfer of a calcium-binding pocket from thermitase protein, PDB entry 1thm, into the first domain of rat CD2 protein, PDB entry 1hng results in new proteins that all exhibit high affinities for terbium and can selectively bind calcium over magnesium

701105