3.4.21.66: Thermitase

This is an abbreviated version!
For detailed information about Thermitase, go to the full flat file.

Reaction

Hydrolysis of proteins, including collagen =

Synonyms

Alkaline protease, EC 3.4.21.14, EC 3.4.4.16, PLLA-degrading enzyme, PrlA, Proteinase, Thermoactinomyces vulgaris serine, proteolysin, Thermoactinomyces vulgaris serine proteinase, Thermophilic Streptomyces serine proteinase, thermostable serine protease

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.66 Thermitase

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Results	in table
7	Activating Compound
131	AA Sequence
2	Application
1	CAS Registry Number
3	Cloned(Commentary)
10	Crystallization (Commentary)
5	General Information
6	General Stability
56	Inhibitors
6	kcat/KM [mM/s]
7	KM Value [mM]
7	Localization
6	Metals/Ions
14	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
47	Organism
4	PDB ID
13	pH Optimum
2	pH Range
4	pH Stability
1	Posttranslational Modification
4	Protein Variants
11	Purification (Commentary)
1	Reaction
1	Reaction Type
47	Reference
6	Source Tissue
6	Specific Activity [micromol/min/mg]
47	Substrates and Products (Substrate)
5	Subunits
14	Synonyms
14	Temperature Optimum [°C]
2	Temperature Range [°C]
10	Temperature Stability [°C]
6	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 3.4.21.66 - Thermitase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

Acetyl-(L-Ala)3 methyl ester + H2O

?

Thermoactinomyces vulgaris

-

best substrate

29565

-

-

?

Anionic protease + H2O

?

Thermoactinomyces vulgaris

-

at pH 8 or 6 and 25°C or 4°C completely hydrolyzed

29545

-

-

?

azocasein + H2O

?

3 entries

Benzyloxycarbonyl-L-Ala-L-Ala-L-Leu 4-nitroanilide + H2O

?

Thermoactinomyces vulgaris

-

-

29546, 29547

-

-

?

Carbobenzoxy-alanine 4-nitrophenyl ester + H2O

?

Streptomyces rectus var. proteolyticus

-

-

29535

-

-

?

casein + H2O

?

4 entries

casein + H2O

hydrolyzed casein

2 entries

Collagen + H2O

Hydrolyzed collagen

Thermoactinomyces vulgaris

-

-

29563

-

-

?

Cry11A endotoxin + H2O

?

Thermoactinomyces vulgaris

-

substrate from Bacillus thuringiensis ssp. israelensis, enzyme hydrolyzes the I28-A29 bond. At increasing concentration of enzyme, additionally lysis of M71-E72 bond

667755

-

-

?

Elastin + H2O

Hydrolyzed elastin

2 entries

Field bean protein + H2O

Hydrolyzed field bean protein

Thermoactinomyces vulgaris

-

-

29565

-

-

?

Gelatin + H2O

?

2 entries

Gelatin + H2O

Hydrolyzed gelatin

Thermoactinomyces vulgaris

-

-

29565

-

-

?

Gluten + H2O

Hydrolyzed gluten

Thermoactinomyces vulgaris

-

-

29565

-

-

?

human skin elastin + H2O

?

Thermoactinomyces vulgaris

-

-

669615

-

-

?

N-Acetyl-L-Tyr ethyl ester + H2O

?

Thermoactinomyces vulgaris

-

-

29549

-

-

?

N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O

N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline

3 entries

N-succinyl-L-Ala-L-Ala-L-Ala 4-nitroanilide + H2O

?

2 entries

oxidized insulin B chain + H2O

?

Coprothermobacter proteolyticus

B5Y6G0

peptidolytic activity, primary cleavage site of proteolysin is located between Ala14 and Leu15. Prolonged incubation of 24 h reveals additional cleavage sites after Phe1, Asn3, Gln4, Leu17, Cys19, Phe24, Phe25, Tyr25, and Lys29, which shows that proteolysin has a relaxed cleavage site specificity

731179

-

-

?

Serum albumin + H2O

Hydrolyzed serum albumin

Thermoactinomyces vulgaris

-

-

29565

-

-

?

Succinyl-Ala-Ala-Phe 4-nitroanilide + H2O

?

Thermoactinomyces vulgaris

-

-

29556

-

-

?

Urea-denatured hemoglobin + H2O

Hydrolyzed urea-denatured hemoglobin

Thermoactinomyces vulgaris

-

-

29565

-

-

?

additional information

?

-

12 entries

azocasein + H2O

?

Coprothermobacter proteolyticus

B5Y6G0

proteinolytic activity

731179

-

-

?

azocasein + H2O

?

Laceyella sacchari

-

-

732871

-

-

?

azocasein + H2O

?

Laceyella sacchari DSM 43353

-

-

732871

-

-

?

casein + H2O

?

Laceyella sacchari

-

strong activity

732254

-

-

?

casein + H2O

?

Laceyella sacchari LP175

-

strong activity

732254

-

-

?

casein + H2O

?

Nocardiopsis alba

-

-

753026

-

-

?

casein + H2O

?

Nocardiopsis alba TATA-5

-

-

753026

-

-

?

casein + H2O

hydrolyzed casein

Streptomyces rectus var. proteolyticus

-

-

29534

-

-

?

casein + H2O

hydrolyzed casein

Thermoactinomyces vulgaris

-

-

29534, 29549, 29565

-

-

?

Elastin + H2O

Hydrolyzed elastin

Thermoactinomyces vulgaris

-

-

29563

-

-

?

Elastin + H2O

Hydrolyzed elastin

Thermoactinomyces vulgaris

-

slowly

29565

-

-

?

Gelatin + H2O

?

Laceyella sacchari

-

strong activity

732254

-

-

?

Gelatin + H2O

?

Laceyella sacchari LP175

-

strong activity

732254

-

-

?

N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O

N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline

Coprothermobacter proteolyticus

B5Y6G0

amidolytic activity

731179

-

-

?

N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O

N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline

Laceyella sacchari

-

-

732871

-

-

?

N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O

N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline

Laceyella sacchari DSM 43353

-

-

732871

-

-

?

N-succinyl-L-Ala-L-Ala-L-Ala 4-nitroanilide + H2O

?

Laceyella sacchari

-

weak activity

732254

-

-

?

N-succinyl-L-Ala-L-Ala-L-Ala 4-nitroanilide + H2O

?

Laceyella sacchari LP175

-

weak activity

732254

-

-

?

additional information

?

-

Coprothermobacter proteolyticus

B5Y6G0

proteolytic activities of cell extracts and purified enzyme samples are monitored on 5% skim milk-LB agar plates or by azocasein assey

731179

-

-

?

additional information

?

-

Coprothermobacter proteolyticus

-

proteolytic activities of cell extracts and purified enzyme samples are monitored on 5% skim milk-LB agar plates or by azocasein assey

731179

-

-

?

additional information

?

-

Laceyella sacchari

-

substrate specificity, overview

732254

-

-

?

additional information

?

-

Laceyella sacchari

-

the enzyme degrades poly(L-lactide) and is a serine protease

732254

-

-

?

additional information

?

-

Laceyella sacchari

-

a thermostable endo-protease with the ability to convert various food relevant substrates into low-molecular weight peptides, endo-catalytic activity

732871

-

-

?

additional information

?

-

Laceyella sacchari DSM 43353

-

a thermostable endo-protease with the ability to convert various food relevant substrates into low-molecular weight peptides, endo-catalytic activity

732871

-

-

?

additional information

?

-

Laceyella sacchari LP175

-

substrate specificity, overview

732254

-

-

?

additional information

?

-

Laceyella sacchari LP175

-

the enzyme degrades poly(L-lactide) and is a serine protease

732254

-

-

?

additional information

?

-

synthetic construct

-

thermitase is characterized by a free cysteine residue near the active site. The enzyme has a preference for large uncharged residues in S1 and S4 (secondary binding pocket). S1–S4 binding sites are rather flat, which may explain broad specificity of the enzyme

681496

-

-

?

additional information

?

-

Thermoactinomyces vulgaris

-

at the active site serine and histidine, cysteine and methionine are essential for activity

29564

-

-

?

additional information

?

-

Thermoactinomyces vulgaris

-

substrate specificity with proteins, amino acid esters, fatty acid esters and amino acid 4-nitroanilides

29565

-

-

?

additional information

?

-

Thermoactinomyces vulgaris

-

thermitase has 5 subsites at the S-site of its active center

29560

-

-

?