3.4.21.66: Thermitase

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For detailed information about Thermitase, go to the full flat file.

Word Map on EC 3.4.21.66

3.4.21.66

subtilisins

3.4.21.14

carlsberg

subtilisin-like

subtilases

subtilisin-type

eglin-c

biotechnology

analysis

Reaction

Hydrolysis of proteins, including collagen =

Synonyms

Alkaline protease, EC 3.4.21.14, EC 3.4.4.16, PLLA-degrading enzyme, PrlA, Proteinase, Thermoactinomyces vulgaris serine, proteolysin, Thermoactinomyces vulgaris serine proteinase, Thermophilic Streptomyces serine proteinase, thermostable serine protease

ECTree

3 Hydrolases

3.4 Acting on peptide bonds (peptidases)

3.4.21 Serine endopeptidases

3.4.21.66 Thermitase

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Results	in table
7	Activating Compound
131	AA Sequence
2	Application
1	CAS Registry Number
3	Cloned(Commentary)
10	Crystallization (Commentary)
5	General Information
6	General Stability
56	Inhibitors
6	kcat/KM [mM/s]
7	KM Value [mM]
7	Localization
6	Metals/Ions
14	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
47	Organism
4	PDB ID
13	pH Optimum
2	pH Range
4	pH Stability
1	Posttranslational Modification
4	Protein Variants
11	Purification (Commentary)
1	Reaction
1	Reaction Type
47	Reference
6	Source Tissue
6	Specific Activity [micromol/min/mg]
47	Substrates and Products (Substrate)
5	Subunits
14	Synonyms
14	Temperature Optimum [°C]
2	Temperature Range [°C]
10	Temperature Stability [°C]
6	Turnover Number [1/s]

Purification

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Purification on EC 3.4.21.66 - Thermitase

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2 entries

autolytic processes may take place during purification

Thermoactinomyces vulgaris

29566

native enzyme 15.3fold to homogeneity from culture supernatant by dialysis, cation exchange chromatography, and again dialysis

Laceyella sacchari

732254

native enzyme from Nocardia alba strain TATA-5 by ammonium sulfate fractionation and anion exchange chromatography

Nocardiopsis alba

753026

native extracellular enzyme from strain KP1 by ammonium sulfate fractionation and gel filtration

Anoxybacillus sp. KP1

753214

recombinant enzyme from culture supernatant by dialysis and hydrophobic interaction chromatography

Laceyella sacchari

732871

recombinant wild-type and mutant N-terminally maltose-binding protein-tagged and C-terminally His6-tagged enzymes from Escherichia coli strain C43(DE3) to near homogeneity from crude cell lysate by heat treatment at 80°C for 3 h, and ultrafiltration

Coprothermobacter proteolyticus

B5Y6G0

731179

single step fine purification

Thermoactinomyces vulgaris

29550