3.4.21.66: Thermitase

This is an abbreviated version!
For detailed information about Thermitase, go to the full flat file.

Word Map on EC 3.4.21.66

3.4.21.66

subtilisins

3.4.21.14

carlsberg

subtilisin-like

subtilases

subtilisin-type

eglin-c

biotechnology

analysis

Reaction

Hydrolysis of proteins, including collagen =

Synonyms

Alkaline protease, EC 3.4.21.14, EC 3.4.4.16, PLLA-degrading enzyme, PrlA, Proteinase, Thermoactinomyces vulgaris serine, proteolysin, Thermoactinomyces vulgaris serine proteinase, Thermophilic Streptomyces serine proteinase, thermostable serine protease

ECTree

3.4 Acting on peptide bonds (peptidases)

3.4.21 Serine endopeptidases

3.4.21.66 Thermitase

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Results	in table
7	Activating Compound
131	AA Sequence
2	Application
1	CAS Registry Number
3	Cloned(Commentary)
10	Crystallization (Commentary)
5	General Information
6	General Stability
56	Inhibitors
6	kcat/KM [mM/s]
7	KM Value [mM]
7	Localization
6	Metals/Ions
14	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
47	Organism
4	PDB ID
13	pH Optimum
2	pH Range
4	pH Stability
1	Posttranslational Modification
4	Protein Variants
11	Purification (Commentary)
1	Reaction
1	Reaction Type
47	Reference
6	Source Tissue
6	Specific Activity [micromol/min/mg]
47	Substrates and Products (Substrate)
5	Subunits
14	Synonyms
14	Temperature Optimum [°C]
2	Temperature Range [°C]
10	Temperature Stability [°C]
6	Turnover Number [1/s]

Inhibitors

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of the Enzyme Summary Page.

Inhibitors on EC 3.4.21.66 - Thermitase

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1,10-phenanthroline

Laceyella sacchari

-

at 5 mM

2,2,5,5-Tetramethylpyrrolin-1-oxyl-3-carbonyl-Ala2-Phe methyl ketone

Thermoactinomyces vulgaris

-

-

2,2,5,5-Tetramethylpyrrolin-1-oxyl-3-carbonyl-Ala3-Phe methyl ketone

Thermoactinomyces vulgaris

-

-

2-mercaptoethanol

Laceyella sacchari

-

slight inhibition

actinomycin

Thermoactinomyces vulgaris

-

SG3, and D

antipain

Thermoactinomyces vulgaris

-

-

bacitracin A

Thermoactinomyces vulgaris

-

-

Benzyloxycarbonyl-(Ala)n-Phe methyl ketone

Thermoactinomyces vulgaris

-

reacts irreversibly in a 1:1 ratio with the enzyme

Benzyloxycarbonyl-Ala2-Phe chloromethyl ketone

Thermoactinomyces vulgaris

-

and the corresponding methyl ketone

benzyloxycarbonyl-Phe chloromethyl ketone

Thermoactinomyces vulgaris

-

-

Benzyloxycarybonyl-(L-Ala)3-L-Phe methyl ketone

Thermoactinomyces vulgaris

-

-

Ca2+

Laceyella sacchari

-

18% inhibition at 1 mM, 85% inhibition at 4 mM

Chloromethyl ketones

Thermoactinomyces vulgaris

-

irreversible

chymostatin

Thermoactinomyces vulgaris

-

-

CTAB

Laceyella sacchari

-

-

Cu2+

Laceyella sacchari

-

8% inhibition at 1 mM, 99% inhibition at 4 mM

Dansyl-(Ala)n-PheCH2Cl

Thermoactinomyces vulgaris

-

n: 1, 2 and 3, and homologous carboxybenzoyl-protected peptides, with a higher inhibitory effect

DTT

Laceyella sacchari

-

slight inhibition at 1-10 mM

EDTA

show

Eglin c

synthetic construct

-

-

EGTA

Laceyella sacchari

-

strong inhibition at 1 mM

Elastinal

Thermoactinomyces vulgaris

-

-

Fe2+

Laceyella sacchari

-

54% inhibition at 1 mM, 80% inhibition at 4 mM

Gramicidin S

Thermoactinomyces vulgaris

-

-

guanidinium hydrochloride

Coprothermobacter proteolyticus

6 M guanidinium hydrochloride gives a 40% reduction of activity after 1 h of incubation at 40°C

H2O2

show

Hg2+

Anoxybacillus sp. KP1

-

-

HgCl2

Thermoactinomyces vulgaris

-

-

Inhibitor from potatoes

Thermoactinomyces vulgaris

-

purification of two stable inhibitors

-

K+

Laceyella sacchari

-

36% inhibition at 1 mM, 53% inhibition at 4 mM

leupeptin

Thermoactinomyces vulgaris

-

-

Methyl ketones of N-acylated peptides

Thermoactinomyces vulgaris

-

reversible

-

Mg2+

Laceyella sacchari

-

54% inhibition at 1 mM, 89% inhibition at 4 mM

Na+

Laceyella sacchari

-

35% inhibition at 1 mM, 54% inhibition at 4 mM

p-chloromercuribenzoate

Thermoactinomyces vulgaris

-

-

Peptide diazomethyl ketones

Thermoactinomyces vulgaris

-

reacts irreversibly in a 1:1 ratio with the enzyme

Peptide methyl ketones

Thermoactinomyces vulgaris

-

kinetic studies on the binding of N-acetylated peptide ketones as substrate analogous

Plysurf A210G

Laceyella sacchari

-

strong inhibition

-

PMSF

show

SDS

Laceyella sacchari

-

strong inhibition

Triton X-100

Laceyella sacchari

-

-

Tween 20

Laceyella sacchari

-

strong inhibition

Tween 80

Laceyella sacchari

-

strong inhibition

Urea

Laceyella sacchari

-

slight inhibition

Zn2+

Laceyella sacchari

-

65% inhibition at 1 mM, 81% inhibition at 4 mM

additional information

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-

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Release 2023.1 (January 2023)