3.4.21.66: Thermitase

This is an abbreviated version!
For detailed information about Thermitase, go to the full flat file.

Reaction

Hydrolysis of proteins, including collagen =

Synonyms

Alkaline protease, EC 3.4.21.14, EC 3.4.4.16, PLLA-degrading enzyme, PrlA, Proteinase, Thermoactinomyces vulgaris serine, proteolysin, Thermoactinomyces vulgaris serine proteinase, Thermophilic Streptomyces serine proteinase, thermostable serine protease

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.66 Thermitase

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Results	in table
7	Activating Compound
131	AA Sequence
2	Application
1	CAS Registry Number
3	Cloned(Commentary)
10	Crystallization (Commentary)
5	General Information
6	General Stability
56	Inhibitors
6	kcat/KM [mM/s]
7	KM Value [mM]
7	Localization
6	Metals/Ions
14	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
47	Organism
4	PDB ID
13	pH Optimum
2	pH Range
4	pH Stability
1	Posttranslational Modification
4	Protein Variants
11	Purification (Commentary)
1	Reaction
1	Reaction Type
47	Reference
6	Source Tissue
6	Specific Activity [micromol/min/mg]
47	Substrates and Products (Substrate)
5	Subunits
14	Synonyms
14	Temperature Optimum [°C]
2	Temperature Range [°C]
10	Temperature Stability [°C]
6	Turnover Number [1/s]

General Stability

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General Stability on EC 3.4.21.66 - Thermitase

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GENERAL STABILITY

ORGANISM

UNIPROT

LITERATURE

Anionic protease, a component which frequently contaminates preparations of routinely isolated cationic protease, stabilizes thermitase

Thermoactinomyces vulgaris

-

29545

Autolysis and thereby inactivation at elevated temperature, at alkaline pH-values and in the absence of added substrate

Thermoactinomyces vulgaris

-

29549

Ca2+ stabilizes against both autolysis and thermal denaturation

Thermoactinomyces vulgaris

-

29549

Irreversible inhibition by chloromethyl ketones causes marked stabilization against thermal denaturation, reversible inhibitors have no influence on stability

Thermoactinomyces vulgaris

-

29561

The stability is significantly improved by 1 M acetate and chloride

Thermoactinomyces vulgaris

-

29563

Unusually tight binding of Ca by thermitase emerges as the most likely single influence responsible for its increased thermostability

Thermoactinomyces vulgaris

-

29553