Any feedback?
Literature summary for 3.4.21.66 extracted from
- Recombinant expression of Laceyella sacchari thermitase in Lactococcus lactis (2013), Protein Expr. Purif., 92, 148-155.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression in Lactococcus lactis strain P170 from vector pAMJ2008 and in strain MG1363, secretion of stable thermitase in an auto-induced fermentation setup at 30°C. The enzyme accumulates in the culture supernatant during batch fermentations and is easily activated at 50°C or by prolonged dialysis | Laceyella sacchari |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Laceyella sacchari |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 8500 | - |
recombinant enzyme, gel filtration, thermitase interactions with the column matrix can delay elution of the protein and lead to underestimation of the molecular size | Laceyella sacchari |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Laceyella sacchari | - |
- |
- |
| Laceyella sacchari DSM 43353 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from culture supernatant by dialysis and hydrophobic interaction chromatography | Laceyella sacchari |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1483 | - |
purified recombinant enzyme, pH 8.0, 60°C | Laceyella sacchari |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| azocasein + H2O | - |
Laceyella sacchari | ? | - |
? | |
| azocasein + H2O | - |
Laceyella sacchari DSM 43353 | ? | - |
? | |
| additional information | a thermostable endo-protease with the ability to convert various food relevant substrates into low-molecular weight peptides, endo-catalytic activity | Laceyella sacchari | ? | - |
? | |
| additional information | a thermostable endo-protease with the ability to convert various food relevant substrates into low-molecular weight peptides, endo-catalytic activity | Laceyella sacchari DSM 43353 | ? | - |
? | |
| N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O | - |
Laceyella sacchari | N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline | - |
? | |
| N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O | - |
Laceyella sacchari DSM 43353 | N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
assay at | Laceyella sacchari |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 80 | activity range, recombinant enzyme | Laceyella sacchari |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
recombinant enzyme, stable up to | Laceyella sacchari |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Laceyella sacchari |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | activity range, recombinant enzyme | Laceyella sacchari |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | purified recombinant enzyme, 50°C, 5 h, over 60% activity | Laceyella sacchari |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
