3.4.21.25: cucumisin

This is an abbreviated version!
For detailed information about cucumisin, go to the full flat file.

Reaction

hydrolysis of proteins with broad specificity =

Synonyms

Cuc m 1, cucumisin, cucumisin homologue, More, protC1, protease C1, religiosin C, soybean protease C1

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.25 cucumisin

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Results	in table
207	AA Sequence
5	Application
1	CAS Registry Number
4	Cloned(Commentary)
2	Crystallization (Commentary)
5	General Stability
1	IC50 Value
42	Inhibitors
5	kcat/KM [mM/s]
3	Ki Value [mM]
33	KM Value [mM]
4	Localization
8	Metals/Ions
10	Molecular Weight [Da]
13	Natural Substrates/ Products (Substrates)
5	Organic Solvent Stability
23	Organism
1	Oxidation Stability
4	PDB ID
10	pH Optimum
8	pH Range
7	pH Stability
2	pI Value
2	Posttranslational Modification
14	Purification (Commentary)
1	Reaction
1	Reaction Type
23	Reference
15	Source Tissue
8	Specific Activity [micromol/min/mg]
7	Storage Stability
126	Substrates and Products (Substrate)
4	Subunits
10	Synonyms
8	Temperature Optimum [°C]
3	Temperature Range [°C]
14	Temperature Stability [°C]
44	Turnover Number [1/s]

Purification

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Purification on EC 3.4.21.25 - cucumisin

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Cucumis melo

-

95353, 95354, 95355

40fold

Cucumis melo

-

95357

60% ammonium sulfate precipitation with 1.0:1.25 ratio of crude extract:t-butanol at pH and temperature of 8.0 and 20°C

Cucumis melo

-

753831

a quick purification method leads to an enzyme fraction with almost only the 67000 Da enzyme

Cucumis melo

-

95358

ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Superdex 200 gel filtration

Ficus religiosa

-

732844

DEAE-Sepharose chromatography, CM-Sepharose chromatography and ammonium sulfate precipitation

Cucumis trigonus

-

670521

gel filtration, SDS-PAGE

Glycine max

-

683714

HiTrap chelating column chromatography and Superdex 200 gel filtration

Cucumis melo

Q39547

754031

HiTrap SP column chromatography and Superdex 200 gel filtration

Cucumis melo

Q39547

732337

Ni-NTA affinity column chromatography

Cucumis melo

-

717062

Ni-Sepharose column chromatography, HiTrap chelating column chromatography, DEAE-Sepharose column chromatography, and Sephacryl S-200 gel filtration

Cucumis melo

-

717802

purified to homogeneity

Cucumis melo

-

95351