Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) cells | Cucumis melo |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ARA12 propeptide | - |
Cucumis melo | |
cucumisin propeptide | potent inhibitor of its mature enzyme, competitive inhibition | Cucumis melo | |
additional information | cucumisin is not inhibited by the subtilisin E propeptide | Cucumis melo | |
RSP1 propeptide | - |
Cucumis melo |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Cucumis melo | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cucumis melo | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose column chromatography, HiTrap chelating column chromatography, DEAE-Sepharose column chromatography, and Sephacryl S-200 gel filtration | Cucumis melo |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Cucumis melo | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutaryl-L-alanyl-L-alanyl-L-prolyl-L-leucyl-4-nitroanilide + H2O | - |
Cucumis melo | glutaryl-L-alanyl-L-alanyl-L-prolyl-L-leucine + 4-nitroaniline | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000062 | - |
cucumisin propeptide | in 150 mM sodium phosphate buffer, pH 7.5 containing 0.2 M NaCl, at 30°C | Cucumis melo | |
0.000062 | - |
ARA12 propeptide | in 150 mM sodium phosphate buffer, pH 7.5 containing 0.2 M NaCl, at 30°C | Cucumis melo | |
0.0001 | - |
RSP1 propeptide | in 150 mM sodium phosphate buffer, pH 7.5 containing 0.2 M NaCl, at 30°C | Cucumis melo |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00002 | - |
pH and temperature not specified in the publication | Cucumis melo | cucumisin propeptide |