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Literature summary for 3.4.21.25 extracted from

  • Nakagawa, M.; Ueyama, M.; Tsuruta, H.; Uno, T.; Kanamaru, K.; Mikami, B.; Yamagata, H.
    Functional analysis of the cucumisin propeptide as a potent inhibitor of its mature enzyme (2010), J. Biol. Chem., 285, 29797-29807.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Cucumis melo

Inhibitors

Inhibitors Comment Organism Structure
ARA12 propeptide
-
Cucumis melo
cucumisin propeptide potent inhibitor of its mature enzyme, competitive inhibition Cucumis melo
additional information cucumisin is not inhibited by the subtilisin E propeptide Cucumis melo
RSP1 propeptide
-
Cucumis melo

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Cucumis melo
-
-

Organism

Organism UniProt Comment Textmining
Cucumis melo
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography, HiTrap chelating column chromatography, DEAE-Sepharose column chromatography, and Sephacryl S-200 gel filtration Cucumis melo

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
Cucumis melo
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutaryl-L-alanyl-L-alanyl-L-prolyl-L-leucyl-4-nitroanilide + H2O
-
Cucumis melo glutaryl-L-alanyl-L-alanyl-L-prolyl-L-leucine + 4-nitroaniline
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000062
-
cucumisin propeptide in 150 mM sodium phosphate buffer, pH 7.5 containing 0.2 M NaCl, at 30°C Cucumis melo
0.000062
-
ARA12 propeptide in 150 mM sodium phosphate buffer, pH 7.5 containing 0.2 M NaCl, at 30°C Cucumis melo
0.0001
-
RSP1 propeptide in 150 mM sodium phosphate buffer, pH 7.5 containing 0.2 M NaCl, at 30°C Cucumis melo

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00002
-
pH and temperature not specified in the publication Cucumis melo cucumisin propeptide