3.4.21.25: cucumisin
This is an abbreviated version!
For detailed information about cucumisin, go to the full flat file.
Word Map on EC 3.4.21.25
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3.4.21.25
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melon
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subtilisin
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subtilisin-like
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sarcocarp
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prince
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merrill
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serping1
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subtilase
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beta-conglycinin
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nutrition
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food industry
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analysis
- 3.4.21.25
- melon
- subtilisin
-
subtilisin-like
- sarcocarp
-
prince
-
merrill
-
serping1
-
subtilase
- beta-conglycinin
- nutrition
- food industry
- analysis
Reaction
hydrolysis of proteins with broad specificity =
Synonyms
Cuc m 1, cucumisin, cucumisin homologue, More, protC1, protease C1, religiosin C, soybean protease C1
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Inhibitors
Inhibitors on EC 3.4.21.25 - cucumisin
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4-(2-aminoethyl)-benzenesulfonyl fluoride
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17% inhibition by 1 mM; i.e. Pefabloc SC
alpha2-Macroglobulin
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38% inhibition by a concentration of 0.001 mM with casein as substrate, and 18% inhibition with succinyl-Ala-Pro-Ala-p-nitroanilide as substrate
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cucumisin propeptide
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potent inhibitor of its mature enzyme, competitive inhibition
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diisopropyl fluorophosphate
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synthesis of denatured diisopropyl phosphorylcucumisin derivatives, which are hydrolyzed by chymotrypsin, to get peptides for amino acid sequence analysis around the reactive serine of the active site
diisopropyl fluorophosphate
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completely inactivated by incubation with 0.5 mM for 20 min
PMSF
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slightly inactivated by 0.1 mM, 11% inhibition by 0.5 mM, 23% inhibition by 1 mM
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cucumisin is not inhibited by the subtilisin E propeptide
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additional information
not inhibited by the C-terminal hepta-peptide of the propeptide (Asn-Glu-Met-Asn-Glu-Leu-His)
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additional information
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not inhibited by the C-terminal hepta-peptide of the propeptide (Asn-Glu-Met-Asn-Glu-Leu-His)
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additional information
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no effect: iodoacetamide, EDTA, PCMB, beta-mercaptoethanol, cysteine, soybean trypsin inhibitor, N-tosyl-L-lysine chloromethyl ketone and N-tosyl-L-phenylalanine chloromethyl ketone
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additional information
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no effect: iodoacetate, EDTA, PCMB, beta-mercaptoethanol, cysteine, N-tosyl-L-phenylalanine chloromethyl ketone and PCMB; no effect of plant proteinous inhibitors of serine proteinases
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additional information
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no inhibition with N-tosyl-L-lysine chloromethyl ketone, N-tosyl-L-phenylalanine chloromethyl ketone, iodoacetate, PCMB, p-chloromercuribenzenesulfonate, E-64, antipain, ovomucoid, EDTA, soybean trypsin inhibitors
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additional information
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no effect of leupeptin, alpha1-antitrypsin, soybean trypsin inhibitor, ovomucoid and aprotinin
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additional information
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no inhibition by EDTA, pepstatin or cysteine protease inhibitors
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additional information
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not inhibited by EDTA, EGTA, iodoacetic acid, 2-phenanthroline, SBTI, and dithiothreitol
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