3.4.21.25: cucumisin

This is an abbreviated version!
For detailed information about cucumisin, go to the full flat file.

hydrolysis of proteins with broad specificity =

Cuc m 1, cucumisin, cucumisin homologue, More, protC1, protease C1, religiosin C, soybean protease C1

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Results	in table
207	AA Sequence
5	Application
1	CAS Registry Number
4	Cloned(Commentary)
2	Crystallization (Commentary)
5	General Stability
1	IC50 Value
42	Inhibitors
5	kcat/KM [mM/s]
3	Ki Value [mM]
33	KM Value [mM]
4	Localization
8	Metals/Ions
10	Molecular Weight [Da]
13	Natural Substrates/ Products (Substrates)
5	Organic Solvent Stability
23	Organism
1	Oxidation Stability
4	PDB ID
10	pH Optimum
8	pH Range
7	pH Stability
2	pI Value
2	Posttranslational Modification
14	Purification (Commentary)
1	Reaction
1	Reaction Type
23	Reference
15	Source Tissue
8	Specific Activity [micromol/min/mg]
7	Storage Stability
126	Substrates and Products (Substrate)
4	Subunits
10	Synonyms
8	Temperature Optimum [°C]
3	Temperature Range [°C]
14	Temperature Stability [°C]
44	Turnover Number [1/s]

General Stability on EC 3.4.21.25 - cucumisin

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20% dimethyl sulfoxide solution, 20°C, stable

8 M urea, pH 9.1, 20 min, no loss of activity

alpha-chymotrypsin has no effect on the activity

incubation with trypsin for 4 h decreases the activity to 30% of the initial activity and after 24 h the enzyme is completely inactivated

the stability of the 67000 Da and the 54000 Da enzyme is almost the same