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3.4.21.25: cucumisin

This is an abbreviated version!
For detailed information about cucumisin, go to the full flat file.

Word Map on EC 3.4.21.25

Reaction

hydrolysis of proteins with broad specificity =

Synonyms

Cuc m 1, cucumisin, cucumisin homologue, More, protC1, protease C1, religiosin C, soybean protease C1

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.25 cucumisin

Temperature Stability

Temperature Stability on EC 3.4.21.25 - cucumisin

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 50
-
the enzyme remains stable for 2 h at 20-50°C. After 2 h at 60, 70, 80, and 90°C, the enzyme shows about 80%, 68%, 15% and 5% residual activity, respectively. The enzyme shows half-lives of 123, 104, 87, 64 and 32 min at 35°C, 40°C, 45°C, 50°C, and 55°C, respectively
20 - 60
20 - 70
25
-
20 h at a pH of 11, 80% of the activity
37
-
24 h, 80% of the activity remains
47
-
20 min at a pH of 11, no loss of activity
50
-
the 54000 Da enzyme stable up to
60
-
1 h at a pH of 11, 65% of the activity
70
-
the 67000 Da enzyme was stable up to
72
-
inactivated after 20 min, ethylene glycol does not stablize, glycerol is effective to some extent
81
melting temperature
90
-
both, the 67000 Da and the 54000 Da enzyme were completely inactivated after 10 min above