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3.4.21.25: cucumisin

This is an abbreviated version!
For detailed information about cucumisin, go to the full flat file.

Word Map on EC 3.4.21.25

Reaction

hydrolysis of proteins with broad specificity =

Synonyms

Cuc m 1, cucumisin, cucumisin homologue, More, protC1, protease C1, religiosin C, soybean protease C1

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.25 cucumisin

Substrates Products

Substrates Products on EC 3.4.21.25 - cucumisin

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-Ala-Ala-Ala-p-nitroanilide + H2O
?
show the reaction diagram
-
1% of the activity with benzyloxycarbonyl-Tyr-nitrophenyl ester
-
-
?
Ala-Ala-Pro-Ala-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
Ala-Ala-Pro-Glu-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
Ala-Ala-Pro-Gly-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
Ala-Ala-Pro-L-diaminopropionic acid-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
Ala-Ala-Pro-Leu-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
Ala-Ala-Pro-Lys-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
Ala-Ala-Pro-Phe-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
Ala-Ala-Pro-Val-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
alpha-mating factor + H2O
?
show the reaction diagram
-
cleaves at one site, Glu-Leu
-
-
?
angiotensin I + H2O
?
show the reaction diagram
-
cleaves at two sites, Tyr-Ile and Phe-His
-
-
?
benzoyl-Tyr-ethyl ester + H2O
benzoyl-Tyr + ethanol
show the reaction diagram
-
24% of the activity with benzyloxycarbonyl-Tyr-nitrophenyl ester
-
-
?
benzyloxycarbonyl-Ala-Ala-Ala-SCH2C6H5 + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Ala-Ala-Arg-SCH2C6H5 + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Ala-Ala-Asp-SCH2C6H5 + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Ala-Ala-Glu-SCH2C6H5 + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Ala-Ala-Leu-SCH2C6H5 + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Ala-Ala-norleucine-SCH2C6H5 + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Ala-Ala-norvaline-SCH2C6H5 + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Ala-Ala-Phe-SCH2C6H5 + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Ala-Ala-Ser-SCH2C6H5 + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Ala-Ala-Val-SCH2C6H5 + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Ala-Asn-SCH2C6H5 + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Glu-Phe + H2O
benzyloxycarbonyl-Glu + Phe
show the reaction diagram
-
weakly hydrolyzed
-
-
?
Benzyloxycarbonyl-Glu-Tyr + H2O
Benzyloxycarbonyl-Glu + Tyr
show the reaction diagram
benzyloxycarbonyl-Tyr-4-nitrophenyl ester + H2O
benzyloxycarbonyl-Tyr + 4-nitrophenyl ester
show the reaction diagram
-
best synthetic substrate
-
-
?
beta-conglycinin storage protein + H2O
?
show the reaction diagram
-
-
-
?
bovine casein + H2O
L-tyrosine + ?
show the reaction diagram
-
-
-
-
?
bovine skimmed milk powder + H2O
?
show the reaction diagram
-
-
-
-
?
casein + H2O
?
show the reaction diagram
Cytochrome c + H2O
?
show the reaction diagram
-
initial cleavage site, Glu-Lys and Ala-Ala
-
-
?
delta sleep-inducing peptide + H2O
?
show the reaction diagram
-
cleaves at one site, Ala-Ser
-
-
?
glutaryl-Ala-Ala-Pro-Leu-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
glutaryl-alanyl-alanyl-prolyl-leucine-4-nitroanilide + H2O
glutaryl-alanyl-alanyl-prolyl-leucine + 4-nitroaniline
show the reaction diagram
-
-
-
?
glutaryl-L-alanyl-L-alanyl-L-prolyl-L-leucyl-4-nitroanilide + H2O
glutaryl-L-alanyl-L-alanyl-L-prolyl-L-leucine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
insulin + H2O
?
show the reaction diagram
-
initial cleavage site, Gln-His
-
-
?
Insulin B-chain + H2O
?
show the reaction diagram
KVEKEESEE + H2O
KVEKE + ESEE
show the reaction diagram
-
-
-
?
L-alanyl-4-nitroanilide + H2O
L-alanine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
L-alanyl-L-alanyl-4-nitroanilide + H2O
L-alanyl-L-alanine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + H2O
L-glutamate + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
L-leucyl-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Leu-Leu-Leu-Leu-Pro-Glu-Ala-Leu + H2O
Leu-Leu-Leu-Leu-Pro-Glu + Ala-Leu
show the reaction diagram
-
-
-
?
Leu-Leu-Leu-Pro-Glu-Ala-Leu + H2O
Leu-Leu-Leu-Pro-Glu + Ala-Leu
show the reaction diagram
-
-
-
?
Leu-Leu-Pro-Ala-Ala-Leu + H2O
Leu-Leu-Pro-Ala + Ala-Leu
show the reaction diagram
-
-
-
?
Leu-Leu-Pro-Arg-Ala-Leu + H2O
Leu-Leu-Pro-Arg + Ala-Leu
show the reaction diagram
-
low activity
-
?
Leu-Leu-Pro-Asn-Ala-Leu + H2O
Leu-Leu-Pro-Asn + Ala-Leu
show the reaction diagram
-
-
-
?
Leu-Leu-Pro-Asp-Ala-Leu + H2O
Leu-Leu-Pro-Asp + Ala-Leu
show the reaction diagram
-
-
-
?
Leu-Leu-Pro-Gln-Ala-Leu + H2O
Leu-Leu-Pro-Gln + Ala-Leu
show the reaction diagram
-
-
-
?
Leu-Leu-Pro-Glu-Ala + H2O
Leu-Leu-Pro-Glu + Ala
show the reaction diagram
-
low activity
-
?
Leu-Leu-Pro-Glu-Ala-Leu + H2O
Leu-Leu-Pro-Glu + Ala-Leu
show the reaction diagram
-
low activity
-
?
Leu-Leu-Pro-Glu-Ala-Leu-Leu + H2O
Leu-Leu-Pro-Glu + Ala-Leu-Leu
show the reaction diagram
-
-
-
?
Leu-Leu-Pro-Glu-Ala-Leu-Leu-Leu + H2O
Leu-Leu-Pro-Glu + Ala-Leu-Leu-Leu
show the reaction diagram
-
-
-
?
Leu-Leu-Pro-Glu-Glu-Leu + H2O
Leu-Leu-Pro-Glu + Glu-Leu
show the reaction diagram
-
low activity
-
?
Leu-Leu-Pro-Glu-Gly-Leu + H2O
Leu-Leu-Pro-Glu + Gly-Leu
show the reaction diagram
-
-
-
?
Leu-Leu-Pro-Glu-Lys-Leu + H2O
Leu-Leu-Pro-Glu + Lys-Leu
show the reaction diagram
-
-
-
?
Leu-Leu-Pro-Glu-Phe-Leu + H2O
Leu-Leu-Pro-Glu + Phe-Leu
show the reaction diagram
-
low activity
-
?
Leu-Leu-Pro-Glu-Ser-Leu + H2O
Leu-Leu-Pro-Glu + Ser-Leu
show the reaction diagram
-
-
-
?
Leu-Leu-Pro-His-Ala-Leu + H2O
Leu-Leu-Pro-His + Ala-Leu
show the reaction diagram
-
-
-
?
Leu-Leu-Pro-Leu-Ala-Leu + H2O
Leu-Leu-Pro-Leu + Ala-Leu
show the reaction diagram
-
-
-
?
Leu-Leu-Pro-Lys-Ala-Leu + H2O
Leu-Leu-Pro-Lys + Ala-Leu
show the reaction diagram
-
low activity
-
?
Leu-Leu-Pro-Met-Ala-Leu + H2O
Leu-Leu-Pro-Met + Ala-Leu
show the reaction diagram
Leu-Leu-Pro-Phe-Ala-Leu + H2O
Leu-Leu-Pro-Phe + Ala-Leu
show the reaction diagram
-
-
-
?
Leu-Leu-Pro-Ser-Ala-Leu + H2O
Leu-Leu-Pro-Ser + Ala-Leu
show the reaction diagram
-
-
-
?
Leu-Leu-Pro-Thr-Ala-Leu + H2O
Leu-Leu-Pro-Thr + Ala-Leu
show the reaction diagram
-
-
-
?
Leu-Leu-Pro-Trp-Ala-Leu + H2O
Leu-Leu-Pro-Trp + Ala-Leu
show the reaction diagram
-
-
-
?
Leu-Pro-Glu-Ala-Leu + H2O
Leu-Pro-Glu + Ala-Leu
show the reaction diagram
-
low activity
-
?
Myoglobin + H2O
?
show the reaction diagram
-
initial cleavage site, Asp-Ile and Glu-Ala
-
-
?
N-acetyl-L-alanyl-L-alanyl-alpha-azaalanine p-nitrophenyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
ovalbumin + H2O
?
show the reaction diagram
-
initial cleavage site, Asn-Ala
-
-
?
peptidyl-p-nitroanilide + H2O
p-nitroaniline + peptide
show the reaction diagram
-
-
-
-
?
Protein + H2O
?
show the reaction diagram
-
-
-
-
?
protein + H2O
hydrolyzed protein
show the reaction diagram
-
-
-
-
?
skim milk + H2O
?
show the reaction diagram
-
-
-
-
?
skim-milk powder + H2O
?
show the reaction diagram
-
milk-clotting activity, same activity as papain, EC 3.4.22.2 and half value of that of ficain, EC 3.4.22.3
-
-
?
Suc-Ala-Ala-Ala-p-nitroanilide + H2O
Suc-Ala-Ala-Ala + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Suc-Ala-Ala-Pro-Leu-p-nitroanilide + H2O
Suc-Ala-Ala-Pro-Leu + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Suc-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
Suc-Ala-Ala-Pro-Phe + p-nitroaniline
show the reaction diagram
-
-
-
-
?
succinyl-Ala-Ala-Ala-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
succinyl-Ala-Ala-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
succinyl-Ala-p-nitroanilide + H2O
succinyl-Ala + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
succinyl-Ala-Pro-Ala-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
superoxide dismutase + H2O
?
show the reaction diagram
-
initial cleavage site, His-Gly and Leu-Ala
-
-
?
TMA-lysozyme BrCN fragment + H2O
?
show the reaction diagram
-
cleaves at two sites, Cys-Lys and Arg-Gly
-
-
?
additional information
?
-