Application | Comment | Organism |
---|---|---|
nutrition | meat tenderizer | Cucumis trigonus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by EDTA, pepstatin or cysteine protease inhibitors | Cucumis trigonus | |
Phenylmethylsulfonylfluoride | strong inhibition | Cucumis trigonus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
SDS-PAGE | Cucumis trigonus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cucumis trigonus | - |
Kachri fruit, Cucumis trigonus Roxburghi | - |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose chromatography, CM-Sepharose chromatography and ammonium sulfate precipitation | Cucumis trigonus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1500 | - |
cell extract, casein substrate | Cucumis trigonus |
3155 | - |
after 2.0fold purification, casein substrate | Cucumis trigonus |
9742 | - |
after 6.5fold purification, casein substrate | Cucumis trigonus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | - |
Cucumis trigonus | ? | - |
? | |
peptidyl-p-nitroanilide + H2O | - |
Cucumis trigonus | p-nitroaniline + peptide | - |
? | |
Suc-Ala-Ala-Ala-p-nitroanilide + H2O | - |
Cucumis trigonus | Suc-Ala-Ala-Ala + p-nitroaniline | - |
? | |
Suc-Ala-Ala-Pro-Leu-p-nitroanilide + H2O | - |
Cucumis trigonus | Suc-Ala-Ala-Pro-Leu + p-nitroaniline | - |
? | |
Suc-Ala-Ala-Pro-Phe-p-nitroanilide + H2O | - |
Cucumis trigonus | Suc-Ala-Ala-Pro-Phe + p-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cucumisin homologue | belongs to the serine protease family | Cucumis trigonus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Cucumis trigonus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | between 20°C and 65°C for 1 h more than 90% activity, 2.5fold and 5fold higher stability at 70°C compared to the activities at 37°C and 20°C, respectively, activity rapidly decreases at temperatures higher than 80°C | Cucumis trigonus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
11 | - |
- |
Cucumis trigonus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | more than 80% of the initial activity after 20 h of incubation at 35°C between pH 4.0 and 10.0, rapid activity loss at pH 2 or pH 12 for 20 h at 35°C, casein substrate | Cucumis trigonus |