3.4.21.114: equine arterivirus serine peptidase
This is an abbreviated version!
For detailed information about equine arterivirus serine peptidase, go to the full flat file.
Word Map on EC 3.4.21.114
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3.4.21.114
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rotavirus
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enterotoxin
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prrsv
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gastroenteritis
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chikungunya
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replicase
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vp4
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chikvs
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rotavirus-infected
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nsp3
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viroporins
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astrovirus
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hp-prrsv
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anti-prrsv
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rv-induced
- 3.4.21.114
- rotavirus
- enterotoxin
- prrsv
- gastroenteritis
-
chikungunya
-
replicase
- vp4
-
chikvs
-
rotavirus-infected
- nsp3
-
viroporins
- astrovirus
-
hp-prrsv
-
anti-prrsv
-
rv-induced
Reaction
cleavage of (Glu/Gln)-/-(Gly/Ser/Ala) in arterivirus replicase translation products ORF1a and ORF1ab =
Synonyms
3C-like serine protease, 3CLSP, equine arteritis virus serine endopeptidase, non-structural protein 4, nonstructural protein 4, nsp4, nsp4 serine protease, nsp4SP, S32.001
ECTree
3.4.21.114 equine arterivirus serine peptidaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.4.21.114 - equine arterivirus serine peptidase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
Dabsyl-Lys-Thr-Ala-Tyr-Phe-Gln-Leu-Glu-Gly-Arg-His-Phe-Glu-EDANS + H2O
Dabsyl-Lys-Thr-Ala-Tyr-Phe-Gln-Leu-Glu + Gly-Arg-His-Phe-Glu-EDANS
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-
-
?
Lys-Asp-Lys-Thr-Ala-Tyr-Phe-Gln-Leu-Glu-Gly-Arg-His-Phe-Thr-Trp + H2O
Lys-Asp-Lys-Thr-Ala-Tyr-Phe-Gln-Leu-Glu + Gly-Arg-His-Phe-Thr-Trp
-
-
-
?
Lys-Thr-Ala-Tyr-Phe-Gln-Leu-Glu-Gly-Arg-His-Phe-Thr-Trp-Tyr-Gln + H2O
Lys-Thr-Ala-Tyr-Phe-Gln-Leu-Glu + Gly-Arg-His-Phe-Thr-Trp-Tyr-Gln
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-
-
?
NSP3'4 protein + H2O
?
recombinantly expressed substrate NSP3'4 covering the last 8 residues of nonstructural protein 3 and the whole amino acid sequence of nonstructural protein 4 mutant S118A
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?
proform caspase-3 + H2O
mature caspase-3 + ?
activation
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-
?
proform caspase-8 + H2O
mature caspase-8 + ?
activation
-
-
?
proform caspase-9 + H2O
mature caspase-9 + ?
activation
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-
?
arterivirus replicase ORF1a + H2O
?
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cleavage sites are Glu1430/Gly and Glu1452/Ser
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?
arterivirus replicase ORF1a + H2O
?
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ORF1a can be cleaved following two alternative pathways. In the majority nsp3-8 precursors are cleaved at the nsp4/5 site yielding nsp3-4 and nsp5-8. The latter product is then cleaved at nsp7/8 only. In the alternative proteolytic cascade the nsp4/5 site remains uncleaved while the nsp5/6 and nsp6/7 sites are processed. nsp3-8 has to interact with cleaved nsp2 to allow processing of the nsp4/5 junction, when nsp2 cofactor is absent the alternative pathway is used
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?
arterivirus replicase ORF1a protein + H2O
?
nsp4 and an unidentified protease is responsible for the cleavage sites nsp2/3, nsp3/4, nsp4/5 and nsp5/6 in the ORF1a protein
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?
arterivirus replicase ORF1a protein + H2O
?
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-
?
arterivirus replicase ORF1ab + H2O
?
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cleavage sites are the junctions at nsp3/4, Nsp4/5, 2 internal nsp5 sites, nsp5/6, p80/p50, p50/p26, p26/p12, enzyme cleaves between Glu and Gly, Glu and Ser or Glu and Lys
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?
arterivirus replicase ORF1ab + H2O
?
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cleavage sites are the junctions at nsp3/4, Nsp4/5, 2 internal nsp5 sites, nsp5/6, p80/p50, p50/p26, p26/p12, enzyme cleaves between Glu and Gly, Glu and Ser or Glu and Lys
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?
arterivirus replicase ORF1b + H2O
?
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protein is processed by nsp4 serine protease
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?
arterivirus replicase ORF1b + H2O
?
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protein is processed by nsp4 serine protease at the following sites: nsp9/10 i.e. p80/p50, cleavage occurs between Glu2370 and Ser, nsp10/11 i.e. p50/p26, cleavage occurs between Gln2837 and Ser, nsp11/12 i.e. nsp26/p12, cleavage between Glu3056 and Gly
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?
arterivirus replicase ORF1b + H2O
?
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protein is processed by nsp4 serine protease
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?
NF-kappaB essential modulator NEMO + H2O
?
-
-
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?
additional information
?
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nsp4 cleaves multiple GluX(Gly/Ser/Ala) sites, X: cleavage site
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?
additional information
?
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nsp4 cleaves multiple GluX(Gly/Ser/Ala) sites, X: cleavage site
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?
additional information
?
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protease has a specificity for Glu/Gly or Glu/Ser substrates
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?
additional information
?
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enzyme is the main viral protease
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?
additional information
?
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the enzyme controls the maturation of the replicase complex. The C-terminal domain has a modulating role in nsp4-mediated autoprocessing of the arterivirus replicase polyproteins
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?
additional information
?
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the enzyme cleaves the cognate nsp6/7- and nsp7/8 site in in vitro synthesized substrates
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?
additional information
?
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nsp4 cleaves multiple GluX(Gly/Ser/Ala) sites, X: cleavage site
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?
additional information
?
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the enzyme shows a 3C-like serine protease activity
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?
additional information
?
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the enzyme shows a 3C-like serine protease activity
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-
?
