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3.4.21.114: equine arterivirus serine peptidase

This is an abbreviated version!
For detailed information about equine arterivirus serine peptidase, go to the full flat file.

Word Map on EC 3.4.21.114

Reaction

cleavage of (Glu/Gln)-/-(Gly/Ser/Ala) in arterivirus replicase translation products ORF1a and ORF1ab =

Synonyms

3C-like serine protease, 3CLSP, equine arteritis virus serine endopeptidase, non-structural protein 4, nonstructural protein 4, nsp4, nsp4 serine protease, nsp4SP, S32.001

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.114 equine arterivirus serine peptidase

Cloned

Cloned on EC 3.4.21.114 - equine arterivirus serine peptidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
fusion protein with maltose-binding protein
GST fusion protein expressed in Escherichia coli BL21(DE3)
large scale production of recombinant nsp4 in Escherichia coli. The nsp4 proteinase is expressed either fused to maltose binding protein or carrying a C-terminal hexahistidine tag
-
ORF1a, fusion protein with glutathione S-transferase
recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)., recombinant expression in HeLa cells