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Literature summary for 3.4.21.114 extracted from

  • Ma, Z.; Wang, Y.; Zhao, H.; Xu, A.T.; Wang, Y.; Tang, J.; Feng, W.H.
    Porcine reproductive and respiratory syndrome virus nonstructural protein 4 induces apoptosis dependent on its 3C-like serine protease activity (2013), PLoS ONE, 8, e69387.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.114

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)., recombinant expression in HeLa cells porcine reproductive and respiratory syndrome virus

Protein Variants

Protein Variants Comment Organism
D64A site-directed mutagenesis, the mutant shows significantly reduced ability to induce apoptosis compared to the wild-type enzyme porcine reproductive and respiratory syndrome virus
H39A site-directed mutagenesis, the mutant shows significantly reduced ability to induce apoptosis compared to the wild-type enzyme porcine reproductive and respiratory syndrome virus
S118A site-directed mutagenesis, the mutant shows significantly reduced ability to induce apoptosis compared to the wild-type enzyme porcine reproductive and respiratory syndrome virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information porcine reproductive and respiratory syndrome virus the enzyme shows a 3C-like serine protease activity ?
-
 ?
proform caspase-3 + H2O porcine reproductive and respiratory syndrome virus activation mature caspase-3 + ?
-
 ?
proform caspase-8 + H2O porcine reproductive and respiratory syndrome virus activation mature caspase-8 + ?
-
 ?
proform caspase-9 + H2O porcine reproductive and respiratory syndrome virus activation mature caspase-9 + ?
-
 ?

Organism

Organism UniProt Comment Textmining
porcine reproductive and respiratory syndrome virus Q91F54
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3). porcine reproductive and respiratory syndrome virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme shows a 3C-like serine protease activity porcine reproductive and respiratory syndrome virus ?
-
 ?
NSP3'4 protein + H2O recombinantly expressed substrate NSP3'4 covering the last 8 residues of nonstructural protein 3 and the whole amino acid sequence of nonstructural protein 4 mutant S118A porcine reproductive and respiratory syndrome virus ?
-
 ?
proform caspase-3 + H2O activation porcine reproductive and respiratory syndrome virus mature caspase-3 + ?
-
 ?
proform caspase-8 + H2O activation porcine reproductive and respiratory syndrome virus mature caspase-8 + ?
-
 ?
proform caspase-9 + H2O activation porcine reproductive and respiratory syndrome virus mature caspase-9 + ?
-
 ?

Synonyms

Synonyms Comment Organism
3C-like serine protease
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 porcine reproductive and respiratory syndrome virus
nonstructural protein 4
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 porcine reproductive and respiratory syndrome virus
nsp4
-
 porcine reproductive and respiratory syndrome virus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
8
-
 assay at porcine reproductive and respiratory syndrome virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at porcine reproductive and respiratory syndrome virus

General Information

General Information Comment Organism
malfunction the ability of nsp4 to induce apoptosis is significantly impaired by His39, Asp64, and Ser118 mutations porcine reproductive and respiratory syndrome virus
additional information residues His39, Asp64, and Ser118 are essential for the enzyme to trigger apoptosis porcine reproductive and respiratory syndrome virus
physiological function porcine reproductive and respiratory syndrome virus nonstructural protein 4 is a critical apoptosis inducer dependent on its serine protease activity, the full-length of nsp4 structure is required for its apoptosis-inducing activity, the enzyme can induce apoptosis in diverse cell lines, e.g. in HeLa cells, overview porcine reproductive and respiratory syndrome virus