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Literature summary for 3.4.21.114 extracted from

  • Wassenaar, A.L.; Spaan, W.J.; Gorbalenya, A.E.; Snijder, E.J.
    Alternative proteolytic processing of the arterivirus replicase ORF1a polyprotein: evidence that NSP2 acts as a cofactor for the NSP4 serine protease (1997), J. Virol., 71, 9313-9322.
    View publication on PubMedView publication on EuropePMC
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
arterivirus replicase ORF1a + H2O equine arteritis virus ORF1a can be cleaved following two alternative pathways. In the majority nsp3-8 precursors are cleaved at the nsp4/5 site yielding nsp3-4 and nsp5-8. The latter product is then cleaved at nsp7/8 only. In the alternative proteolytic cascade the nsp4/5 site remains uncleaved while the nsp5/6 and nsp6/7 sites are processed. nsp3-8 has to interact with cleaved nsp2 to allow processing of the nsp4/5 junction, when nsp2 cofactor is absent the alternative pathway is used ?
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Organism

Organism UniProt Comment Textmining
equine arteritis virus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arterivirus replicase ORF1a + H2O cleavage sites are Glu1430/Gly and Glu1452/Ser equine arteritis virus ?
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arterivirus replicase ORF1a + H2O ORF1a can be cleaved following two alternative pathways. In the majority nsp3-8 precursors are cleaved at the nsp4/5 site yielding nsp3-4 and nsp5-8. The latter product is then cleaved at nsp7/8 only. In the alternative proteolytic cascade the nsp4/5 site remains uncleaved while the nsp5/6 and nsp6/7 sites are processed. nsp3-8 has to interact with cleaved nsp2 to allow processing of the nsp4/5 junction, when nsp2 cofactor is absent the alternative pathway is used equine arteritis virus ?
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additional information protease has a specificity for Glu/Gly or Glu/Ser substrates equine arteritis virus ?
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Synonyms

Synonyms Comment Organism
nonstructural protein 4
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 equine arteritis virus
nsp4
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 equine arteritis virus
S32.001 Merops ID equine arteritis virus