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Literature summary for 3.4.21.114 extracted from

  • Snijder, E.J.; Wassenaar, A.L.; van Dinten, L.C.; Spaan, W.J.; Gorbalenya, A.E.
    The arterivirus nsp4 protease is the prototype of a novel group of chymotrypsin-like enzymes, the 3C-like serine proteases (1996), J. Biol. Chem., 271, 4864-4871.
    View publication on PubMed
Search for more literature for 3.4.21.114

Cloned(Commentary)

Cloned (Comment) Organism
-
 equine arteritis virus

Protein Variants

Protein Variants Comment Organism
D1129E mutant is able to cleave the Nsp4/5 site with wild-type efficiently but does not process the Nsp3/4 and Nsp5/6 junctions equine arteritis virus
D1129K no Nsp3456 cleavage equine arteritis virus
D1129V no Nsp3456 cleavage equine arteritis virus
H1103G complete inhibition of Nsp3456 processing equine arteritis virus
H1103R complete inhibition of Nsp3456 processing equine arteritis virus
H1198L completely abolished processing of Nsp4/5 and Nsp5/6 equine arteritis virus
H1198R completely abolished processing of Nsp4/5 and Nsp5/6 equine arteritis virus
H1198Y completely abolished processing of Nsp4/5 and Nsp5/6 equine arteritis virus
S1184C mutation has no effect on Nsp3456 processing equine arteritis virus
S1184F mutation has no effect on Nsp3456 processing equine arteritis virus
S1184I mutation has no effect on Nsp3456 processing equine arteritis virus
S1184Y mutation has no effect on Nsp3456 processing equine arteritis virus
T1179D completely abolished processing of Nsp4/5 and Nsp5/6 equine arteritis virus
T1179G reduced cleavage of Nsp4/5, no cleavage of Nsp5/6 site equine arteritis virus
T1179N retains wild-type activity towards Nsp4/5, cleaves Nsp5/6 with increased efficiency equine arteritis virus
T1179S reduced cleavage of Nsp4/5, no cleavage of Nsp5/6 site equine arteritis virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
 calculated from amino acid sequence equine arteritis virus
31000
-
 SDS-PAGE equine arteritis virus

Organism

Organism UniProt Comment Textmining
equine arteritis virus P19811 Bucyrus strain
-
equine arteritis virus Bucyrus P19811 Bucyrus strain
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification replicase of EAV processed by at least 3 viral proteases, resulting in the generation of non-structural proteins Nsp1-6 equine arteritis virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arterivirus replicase ORF1a protein + H2O
-
 equine arteritis virus ?
-
 ?
arterivirus replicase ORF1a protein + H2O
-
 equine arteritis virus Bucyrus ?
-
 ?
additional information nsp4 cleaves multiple GluX(Gly/Ser/Ala) sites, X: cleavage site equine arteritis virus ?
-
 ?
additional information nsp4 cleaves multiple GluX(Gly/Ser/Ala) sites, X: cleavage site equine arteritis virus Bucyrus ?
-
 ?

Synonyms

Synonyms Comment Organism
nsp4
-
 equine arteritis virus