3.4.17.11: glutamate carboxypeptidase

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For detailed information about glutamate carboxypeptidase, go to the full flat file.

Reaction

release of C-terminal glutamate residues from a wide range of N-acylating moieties, including peptidyl, aminoacyl, benzoyl, benzyloxycarbonyl, folyl and pteroyl groups =

Synonyms

carboxypeptidase G, carboxypeptidase G1, carboxypeptidase G2, CPDG2, CPG2, folate hydrolase G2, glucarpidase, glutamate carboxypeptidase, glutamyl carboxypeptidase, N-pteroyl-L-glutamate hydrolase, pteroylmonoglutamic acid hydrolase G2

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.17 Metallocarboxypeptidases

                3.4.17.11 glutamate carboxypeptidase

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Results	in table
1463	AA Sequence
17	Application
3	CAS Registry Number
7	Cloned(Commentary)
3	Crystallization (Commentary)
273	Disease/ Diagnostics
2	General Stability
2	IC50 Value
33	Inhibitors
27	KM Value [mM]
1	Localization
15	Metals/Ions
9	Molecular Weight [Da]
21	Natural Substrates/ Products (Substrates)
26	Organism
20	PDB ID
10	pH Optimum
1	pH Range
1	Posttranslational Modification
11	Protein Variants
11	Purification (Commentary)
1	Reaction
3	Reaction Type
26	Reference
1	Renatured (Commentary)
10	Source Tissue
6	Specific Activity [micromol/min/mg]
4	Storage Stability
98	Substrates and Products (Substrate)
5	Subunits
45	Synonyms
5	Temperature Optimum [°C]
1	Temperature Range [°C]
12	Temperature Stability [°C]
15	Turnover Number [1/s]

Cloned

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Cloned on EC 3.4.17.11 - glutamate carboxypeptidase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expression in Escherichia coli BL21(DE3)

Pseudomonas sp. RS-16

P06621

752914, 753264, 755292

for expression in Salmonella typhimurium cells using the vector pTrc99A

Pseudomonas sp.

-

697374

high-yield expression in Escherichia coli, expression of the full-length enzyme, as well as protein fragments corresponding to the individual catalytic and dimerization domains

Pseudomonas sp. RS-16

P06621

755301

into a binary vector for Agrobacterium tumefaciens-mediated transformation

Oryza sativa

Q852M4

700756

overexpression of a codon-optimized gene in Escherichia coli, vector pET28a, the enzyme is expressed to about 60% of the total host protein and the purification of the recombinant His-tagged protein could be achieved in a single step by Ni2+ charged column chromatography

Pseudomonas sp.

P06621

710483