Cloned (Comment) | Organism |
---|---|
overexpression of a codon-optimized gene in Escherichia coli, vector pET28a, the enzyme is expressed to about 60% of the total host protein and the purification of the recombinant His-tagged protein could be achieved in a single step by Ni2+ charged column chromatography | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Pseudomonas sp. | |
additional information | Mg2+ inhibits the activity of the recombinant enzyme; Mn2+ inhibits the activity of the recombinant enzyme | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Mg2+ inhibits the activity of the recombinant enzyme | Pseudomonas sp. | |
additional information | Mn2+ inhibits the activity of the recombinant enzyme | Pseudomonas sp. | |
Zn2+ | zinc dependant | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
SDS-PAGE | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | P06621 | - |
- |
Purification (Comment) | Organism |
---|---|
as His-tagged protein in a single step by Ni2+ charged column chromatography | Pseudomonas sp. |
Renatured (Comment) | Organism |
---|---|
Refolding is initiated by rapid 25fold dilution of the denatured protein into refolding buffer (100 mM Tris-HCl, pH 8.5, containing 0.1 mM EDTA and 0.5 M arginine) | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methotrexate + H2O | - |
Pseudomonas sp. | 4-amino-4-deoxy-N10-methylpteroic acid + L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
carboxypeptidase G2 | - |
Pseudomonas sp. |
CPG2 | - |
Pseudomonas sp. |
glucarpidase | - |
Pseudomonas sp. |