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135
(4-nitro)Phe-Pro-HN-CH2-CH2-NH-o-aminobenzoyl
-
-
20
(4-nitro)Phe-Pro-Pro-HN-CH2-CH2-NH-o-aminobenzoyl
-
-
7.3 - 95
Abz-L-Lys-L-Pro-L-Pro-4-nitroanilide
125
Arg-Pro-Pro-benzylamide
-
-
15 - 48
Arg-Pro-Pro-Gly-Phe
11 - 30
Arg-Pro-Pro-Gly-Phe-Ser
16 - 41
Arg-Pro-Pro-Gly-Phe-Ser-Pro
13 - 39
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe
12 - 26
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
47.2
Gly-Pro-Pro-p-nitroanilide
-
-
1.5 - 487
L-Ala-L-Pro-4-nitroanilide
0.77
L-Ala-L-Pro-L-Ala
wild-type, pH 8.1, 37°C
7.7
L-Arg-L-Pro-L-Pro
-
wild type enzyme in 100 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 37°C
17
L-Arg-L-Pro-L-Pro-Gly
-
-
-
541
L-Met-L-Pro
pH 5.0, 80°C
58
L-Tyr-L-Pro-L-Phe-NH2
-
-
-
3.1
Substance P
pH 8.2, 37°C, recombinant wild-type enzyme
1.4
Tyr-Ala-Ala
pH 7.5, 40°C
14
Tyr-Pro-Ala
pH 7.5, 40°C
40
Tyr-Pro-Leu-Gly-NH2
-
-
32
Tyr-Pro-Phe-Pro-Gly
-
-
26
Tyr-Pro-Phe-Pro-Gly-Pro-Ile
-
-
7.3
Abz-L-Lys-L-Pro-L-Pro-4-nitroanilide
mutant H350, pH 8.1, 37°C
95
Abz-L-Lys-L-Pro-L-Pro-4-nitroanilide
wild-type, pH 8.1, 37°C
29
Arg-Pro-Ala
pH 8.0, 55°C, recombinant mutant D53A
101
Arg-Pro-Ala
pH 8.0, 55°C, recombinant mutant R55A
152
Arg-Pro-Ala
pH 8.0, 55°C, recombinant mutant Y56A
240
Arg-Pro-Ala
pH 8.0, 55°C, recombinant wild-type
49
Arg-Pro-Pro
-
-
180
Arg-Pro-Pro
-
in presence of 4 mM Mn2+
230
Arg-Pro-Pro
-
without 4 mM Mn2+
15
Arg-Pro-Pro-Gly-Phe
-
-
48
Arg-Pro-Pro-Gly-Phe
-
-
11
Arg-Pro-Pro-Gly-Phe-Ser
-
-
30
Arg-Pro-Pro-Gly-Phe-Ser
-
-
16
Arg-Pro-Pro-Gly-Phe-Ser-Pro
-
-
41
Arg-Pro-Pro-Gly-Phe-Ser-Pro
-
-
13
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe
-
-
39
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe
-
-
12
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
-
-
26
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
-
-
0.17
bradykinin
-
37°C, mutant H519K
0.17
bradykinin
-
mutant R404A, pH 7.5, 37°C
0.24
bradykinin
-
mutant R404K, pH 7.5, 37°C
0.9
bradykinin
pH 7.5, 37°C, recombinant mutant R535A
1.18
bradykinin
-
37°C, wild-type enzyme
1.8
bradykinin
pH 7.5, 37°C, recombinant mutant Y527F
1.82
bradykinin
-
37°C, mutant H519L
2
bradykinin
pH 7.5, 37°C, recombinant mutant R535A, with 0.1 mM guanidine hydrochloride
2.94
bradykinin
-
37°C, mutant H519L
3.13
bradykinin
pH 8.2, 37°C, recombinant wild-type enzyme
3.2
bradykinin
pH 7.5, 37°C, recombinant mutant R535A, with 1.0 mM guanidine hydrochloride
3.8
bradykinin
-
wild type enzyme in 100 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 37°C
4.2
bradykinin
-
mutant Y387F, pH 7.5, 37°C
4.48
bradykinin
pH 8.2, 37°C, recombinant wild-type enzyme
6.08
bradykinin
-
37°C, wild-type enzyme
8.3
bradykinin
pH 7.5, 37°C, recombinant mutant R535A, with 10 mM guanidine hydrochloride
11
bradykinin
pH 7.5, 37°C, recombinant wild-type enzyme
13.8
bradykinin
-
mutant W88L, pH 7.5, 37°C
16
bradykinin
-
pH 7.5, 37°C
57
bradykinin
-
mutant R153L/R370L, pH 7.5, 37°C
69
bradykinin
-
wild-type, pH 7.5, 37°C
70
bradykinin
-
mutant R153W, pH 7.5, 37°C
72
bradykinin
-
mutant R153A, pH 7.5, 37°C
87
bradykinin
-
mutant R153W/R370L, pH 7.5, 37°C
88
bradykinin
-
pH 6.5, 37°C
102
bradykinin
-
mutant R370L, pH 7.5, 37°C
109
bradykinin
-
mutant R153L, pH 7.5, 37°C
160
bradykinin
-
pH 5.5, 37°C
5.4
FPHFD
-
pH 5.5, 37°C
48
Gly-Pro-Hyp
-
-
69
Gly-Pro-Hyp
-
in presence of 4 mM Mn2+
1.5
L-Ala-L-Pro-4-nitroanilide
-
chimera th-sl cAPP, pH 7.0, 30°C
1.5
L-Ala-L-Pro-4-nitroanilide
chimera th-sl cAPP, pH 7.0, 30°C
6
L-Ala-L-Pro-4-nitroanilide
-
chimera sl-th cAPP, pH 8.2, 30°C
6
L-Ala-L-Pro-4-nitroanilide
chimera sl-th cAPP, pH 8.2, 30°C
45.7
L-Ala-L-Pro-4-nitroanilide
-
recombinant enzyme, pH 7.4, 30°C
398
L-Ala-L-Pro-4-nitroanilide
recombinant enzyme, pH 7.4, 30°C
487
L-Ala-L-Pro-4-nitroanilide
native enzyme, pH 7.4, 30°C
1.5
Met-Ala-Ala
pH 8.0, 55°C, recombinant wild-type
5
Met-Ala-Ala
pH 8.0, 55°C, recombinant wild-type
10.5
Met-Ala-Ala
pH 8.0, 55°C, recombinant wild-type
18
Met-Pro
pH 8.0, 55°C, recombinant wild-type
33
Met-Pro
pH 8.0, 55°C, recombinant wild-type
97
Met-Pro
pH 8.0, 55°C, recombinant wild-type
2 - 8
Met-Pro-Ala
pH 8.0, 55°C, recombinant wild-type
25.7
Met-Pro-Ala
pH 7.5, 40°C
35
Met-Pro-Ala
pH 8.0, 55°C, recombinant mutant D53A
126
Met-Pro-Ala
pH 8.0, 55°C, recombinant wild-type
140
Met-Pro-Ala
pH 8.0, 55°C, recombinant mutant Y56A
144
Met-Pro-Ala
pH 8.0, 55°C, recombinant mutant R55A
242
Met-Pro-Ala
pH 8.0, 55°C, recombinant wild-type
12
YPWTQ
-
pH 5.5, 37°C
3.4.11.9 Xaa-Pro aminopeptidase
results (
results (
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