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3.4.11.9: Xaa-Pro aminopeptidase

This is an abbreviated version!
For detailed information about Xaa-Pro aminopeptidase, go to the full flat file.

Word Map on EC 3.4.11.9

Reaction

release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide =

Synonyms

aminoacylproline aminopeptidase, aminopeptidase P, aminopeptidase P-like enzyme, aminopeptidase P1, aminopeptidase, aminoacylproline, AMPP, AP-P, APaseP, APP, APP1, APPro, DAP-P, dapUm, Dr-smAPP, Ec-smAPP, ecAPP, hAPP1, hcAMPP, Icp55, LeAPP1, LeAPP2, M24B peptidase, mAmP, Membrane-bound AmP, Membrane-bound APP, membrane-bound proline-specific APaseP, More, Mt-smAPP, Pa-PepP, PEPP, PepQ, peptidase PepQ, PepX aminopeptidase, PfAPP, proline aminopeptidase, sll0136, small aminopeptidase-P, TgAPP, TvMP50, X-Pro aminopeptidase, X-prolyl aminopeptidase, X-prolyl aminopeptidase 2, X-prolyl aminopeptidase 3, X-prolyl peptidase, X-prolyl-dipeptidyl aminopeptidase, Xaa-Pro aminopeptidase, Xaa-Pro aminopeptidase-1, Xaa-Pro aminopeptidase-2, Xaa-Pro dipeptidase, XPD, XpmA, XPNEP2, XPNPEP-1, XPNPEP-2, XPNPEP1, XPNPEP2, XPNPEP3, YpdF

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.11 Aminopeptidases
                3.4.11.9 Xaa-Pro aminopeptidase

Cloned

Cloned on EC 3.4.11.9 - Xaa-Pro aminopeptidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned in Escherichia coli
cloning, DNA and amino acid sequence determination and analysis, and functional expression of open reading frame W03H9.4 from EST yk91g4 in Caenorhabditis elegans as GFP-fusion protein and as His-tagged enzyme in Escherichia coli
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DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli
DNA and amino acid sequence determination and analysis, expression in Escherichia coli BL21(DE3) as His-tagged enzyme, and in COS-1 cells
expressed in Escherichia coli as a His-tagged fusion protein
expressed in Escherichia coli BL21(DE3) cells
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expressed in Pichia pastoris
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expressed in Streptomyces lividans using the pTONA5a plasmid
expression as a fusion with the yellow fluorescent protein allele Citrine or with hemagglutinin tag in Plasmodium falciparum
expression in Escherichia coli
expression in Escherichia coli BL21(DE3). Full-length Icp55 construct can not be expressed in the soluble fraction. Two truncated versions, Icp55t1 with 42 amino acid N-terminal truncation and Icp55t2 with 57 amino acid N-terminal truncation, are successfully expressed in soluble forms. All activity assays for Icp55 are reported for Icp55t1 construct
gene ampp, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21
gene LeAPP1, DNA and amino acid sequence determination and analysis
gene LeAPP2, DNA and amino acid sequence determination and analysis, expression in Escherichia coli in N-terminal fusion with GST, a mutant form lacking 11 amino acids from the N-terminus is not active
gene pepP, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene sll0136, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS
gene TgApp, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21
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gene XPNPEP-1, quantitative enzyme expression analysis
gene XPNPEP-2, quantitative enzyme expression analysis
gene XPNPEP1, recombinant expression of wild-type and mutant cytosolic aminopeptidases P (hcAMPP, residues M1 to H623), with a thrombin-cleavable N-terminal His-tag in Escherichia coli strain Rosetta (DE3)
overexpression in Escherichia coli
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recombinant expression in Escherichia coli as a His-tagged fusion protein
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recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
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recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Star (DE3)
recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS
recombinant expression of wild-type and mutant His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS
sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli
transient expression of wild-type and mutants in COS-1 cells
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