Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glutathione | activates the enzyme in presence of Mn2+ or Co2+, and slightly in presence of Zn2+ | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in Escherichia coli BL21(DE3) as His-tagged enzyme, and in COS-1 cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | complete inhibition at 0.1 mM | Homo sapiens | |
apstatin | complete inhibition at 0.1 mM | Homo sapiens | |
Ca2+ | 83.1% inhibition at 1 mM | Homo sapiens | |
Co2+ | inhibitory at 1 mM | Homo sapiens | |
Cu2+ | complete inhibition at 1 mM | Homo sapiens | |
dithiothreitol | - |
Homo sapiens | |
EDTA | 49% inhibition at 1 mM | Homo sapiens | |
glutathione | 10% inhibition at 1 mM in absence of cations | Homo sapiens | |
Mg2+ | 38.2% inhibition at 1 mM | Homo sapiens | |
additional information | no significant inhibition by amastatin and enalaprilat | Homo sapiens | |
Ni2+ | 93.5% inhibition at 1 mM | Homo sapiens | |
Zn2+ | complete inhibition at 0.1 mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.077 | - |
Substance P | pH 8.2, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.101 | - |
bradykinin | pH 8.2, 37°C, recombinant wild-type enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | in a metal:protein ratio of 0.11:1, slightly activates the enzyme at 0.01-0.1 mM, 2.8fold activation at 1 mM in presence of 1 mM glutathione | Homo sapiens | |
Fe2+ | in a metal:protein ratio of 0.07:1 | Homo sapiens | |
Mn2+ | in a metal:protein ratio of 1:1, activates the enzyme 2.80fold at 0.1 mM with substrate substance P, maximal at 0.3 mM, 4.6fold activation at 1 mM in presence of 1 mM glutathione | Homo sapiens | |
Zn2+ | in a metal:protein ratio of 0.11:1 | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71000 | - |
2 * 71000, recombinant enzyme, SDS-PAGE | Homo sapiens |
140000 | - |
approximately, gel filtration and native PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NQW7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Ecscherichia coli | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leukocyte | - |
Homo sapiens | - |
platelet | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bradykinin + H2O | hydrolysis of the N-terminal Arg1-Pro bond | Homo sapiens | Arg + des-Arg-bradykinin | - |
? | |
additional information | no activity with Gly-Pro-hydroxyPro | Homo sapiens | ? | - |
? | |
substance P + H2O | - |
Homo sapiens | Arg + des-Arg-substance P | - |
? | |
substance P + H2O | - |
Homo sapiens | L-Arg + PKPQQFFGLM | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 71000, recombinant enzyme, SDS-PAGE | Homo sapiens |
More | interaction of the subunits can not be disrupted by 2-mercaptoethanol, 1 M NaCl, 1% Triton X-100, and 4 mM CHAPS | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
aminopeptidase P | - |
Homo sapiens |
AP-P | - |
Homo sapiens |
More | the enzyme belongs to the peptidase family M24 | Homo sapiens |
X-Pro aminopeptidase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.1 | - |
Substance P | pH 8.2, 37°C, recombinant wild-type enzyme | Homo sapiens | |
3.13 | - |
bradykinin | pH 8.2, 37°C, recombinant wild-type enzyme | Homo sapiens | |
4.48 | - |
bradykinin | pH 8.2, 37°C, recombinant wild-type enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
recombinant enzyme expressed in Escherichia coli | Homo sapiens |