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Literature summary for 3.4.11.9 extracted from

  • Cottrell, G.S.; Hooper, N.M.; Turner, A.J.
    Cloning, expression, and characterization of human cytosolic aminopeptidase P: a single manganese(II)-dependent enzyme (2000), Biochemistry, 39, 15121-15128.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
glutathione activates the enzyme in presence of Mn2+ or Co2+, and slightly in presence of Zn2+ Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression in Escherichia coli BL21(DE3) as His-tagged enzyme, and in COS-1 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline complete inhibition at 0.1 mM Homo sapiens
apstatin complete inhibition at 0.1 mM Homo sapiens
Ca2+ 83.1% inhibition at 1 mM Homo sapiens
Co2+ inhibitory at 1 mM Homo sapiens
Cu2+ complete inhibition at 1 mM Homo sapiens
dithiothreitol
-
Homo sapiens
EDTA 49% inhibition at 1 mM Homo sapiens
glutathione 10% inhibition at 1 mM in absence of cations Homo sapiens
Mg2+ 38.2% inhibition at 1 mM Homo sapiens
additional information no significant inhibition by amastatin and enalaprilat Homo sapiens
Ni2+ 93.5% inhibition at 1 mM Homo sapiens
Zn2+ complete inhibition at 0.1 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.077
-
Substance P pH 8.2, 37°C, recombinant wild-type enzyme Homo sapiens
0.101
-
bradykinin pH 8.2, 37°C, recombinant wild-type enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ in a metal:protein ratio of 0.11:1, slightly activates the enzyme at 0.01-0.1 mM, 2.8fold activation at 1 mM in presence of 1 mM glutathione Homo sapiens
Fe2+ in a metal:protein ratio of 0.07:1 Homo sapiens
Mn2+ in a metal:protein ratio of 1:1, activates the enzyme 2.80fold at 0.1 mM with substrate substance P, maximal at 0.3 mM, 4.6fold activation at 1 mM in presence of 1 mM glutathione Homo sapiens
Zn2+ in a metal:protein ratio of 0.11:1 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
2 * 71000, recombinant enzyme, SDS-PAGE Homo sapiens
140000
-
approximately, gel filtration and native PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NQW7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Ecscherichia coli Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
leukocyte
-
Homo sapiens
-
platelet
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bradykinin + H2O hydrolysis of the N-terminal Arg1-Pro bond Homo sapiens Arg + des-Arg-bradykinin
-
?
additional information no activity with Gly-Pro-hydroxyPro Homo sapiens ?
-
?
substance P + H2O
-
Homo sapiens Arg + des-Arg-substance P
-
?
substance P + H2O
-
Homo sapiens L-Arg + PKPQQFFGLM
-
?

Subunits

Subunits Comment Organism
dimer 2 * 71000, recombinant enzyme, SDS-PAGE Homo sapiens
More interaction of the subunits can not be disrupted by 2-mercaptoethanol, 1 M NaCl, 1% Triton X-100, and 4 mM CHAPS Homo sapiens

Synonyms

Synonyms Comment Organism
aminopeptidase P
-
Homo sapiens
AP-P
-
Homo sapiens
More the enzyme belongs to the peptidase family M24 Homo sapiens
X-Pro aminopeptidase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1
-
Substance P pH 8.2, 37°C, recombinant wild-type enzyme Homo sapiens
3.13
-
bradykinin pH 8.2, 37°C, recombinant wild-type enzyme Homo sapiens
4.48
-
bradykinin pH 8.2, 37°C, recombinant wild-type enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
recombinant enzyme expressed in Escherichia coli Homo sapiens