Protein Variants | Comment | Organism |
---|---|---|
R153A | enzymic activity similar to wild-type | Escherichia coli |
R153L | enzymic activity similar to wild-type | Escherichia coli |
R153L/R370L | decrease in Km-value | Escherichia coli |
R153W | enzymic activity similar to wild-type | Escherichia coli |
R153W/R370L | decrease in Km-value | Escherichia coli |
R370L | enzymic activity similar to wild-type | Escherichia coli |
R404A | decrease both in KM- and kcat-value | Escherichia coli |
R404K | decrease in kcat-value | Escherichia coli |
W88L | enzymic activity similar to wild-type | Escherichia coli |
Y387F | decrease in kcat-value | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.047 | - |
bradykinin | mutant R404A, pH 7.5, 37°C | Escherichia coli | |
0.16 | - |
bradykinin | mutant R153W/R370L, pH 7.5, 37°C | Escherichia coli | |
0.18 | - |
bradykinin | mutant R153L/R370L, pH 7.5, 37°C | Escherichia coli | |
0.21 | - |
bradykinin | mutant R370L, pH 7.5, 37°C | Escherichia coli | |
0.41 | - |
bradykinin | mutant R404K, pH 7.5, 37°C | Escherichia coli | |
0.42 | - |
bradykinin | mutant R153A, pH 7.5, 37°C | Escherichia coli | |
0.43 | - |
bradykinin | mutant R153W, pH 7.5, 37°C | Escherichia coli | |
0.51 | - |
bradykinin | mutant R153L, pH 7.5, 37°C | Escherichia coli | |
0.78 | - |
bradykinin | wild-type, pH 7.5, 37°C | Escherichia coli | |
0.84 | - |
bradykinin | mutant Y387F, pH 7.5, 37°C | Escherichia coli | |
1.02 | - |
bradykinin | mutant W88L, pH 7.5, 37°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | wild-type and mutat R404A, binding of Mn2+ with a stoichiometry of 2 per monomer | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide | R404 participates in proton relay and in the hydrogen bond network | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.17 | - |
mutant R404A, pH 7.5, 37°C | Escherichia coli |
0.95 | - |
mutant R404A, presence of guanidine, pH 7.5, 37°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bradykinin + H2O | - |
Escherichia coli | des-Arg-bradykinin + L-Arg | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
bradykinin | mutant R404A, pH 7.5, 37°C | Escherichia coli | |
0.24 | - |
bradykinin | mutant R404K, pH 7.5, 37°C | Escherichia coli | |
4.2 | - |
bradykinin | mutant Y387F, pH 7.5, 37°C | Escherichia coli | |
13.8 | - |
bradykinin | mutant W88L, pH 7.5, 37°C | Escherichia coli | |
57 | - |
bradykinin | mutant R153L/R370L, pH 7.5, 37°C | Escherichia coli | |
69 | - |
bradykinin | wild-type, pH 7.5, 37°C | Escherichia coli | |
70 | - |
bradykinin | mutant R153W, pH 7.5, 37°C | Escherichia coli | |
72 | - |
bradykinin | mutant R153A, pH 7.5, 37°C | Escherichia coli | |
87 | - |
bradykinin | mutant R153W/R370L, pH 7.5, 37°C | Escherichia coli | |
102 | - |
bradykinin | mutant R370L, pH 7.5, 37°C | Escherichia coli | |
109 | - |
bradykinin | mutant R153L, pH 7.5, 37°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
wild-type and mutant R404A | Escherichia coli |