Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces lividans |
expression in Escherichia coli | Streptomyces murinus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of chimeric enzymes th-sl cAPP, in which half of the N-terminal domain is derived from Streptomyces costaricanus and the rest is Streptomyces lividans isoform II and sl-th cAPP, in which half of the N-terminal domain is Streptomyces lividans isoform II and the rest is Streptomyces costaricanus. Both chimera are activated by Zn2+. Th-sl cAPP and sl-th cAPP, respectively, form a tetramer and a dimer. The pH dependence and pKa values of th-sl cAPP were almost identical to those of Streptomyces costaricanus. In contrast, sl-th cAPP showed curves and pKa values that resembled those of Streptomyces lividans isoform II | Streptomyces lividans |
additional information | construction of chimeric enzymes th-sl cAPP, in which half of the N-terminal domain is derived from Streptomyces costaricanus and the rest is Streptomyces lividans isoform II and sl-th cAPP, in which half of the N-terminal domain is Streptomyces lividans isoform II and the rest is Streptomyces costaricanus. Both chimera are activated by Zn2+. Th-sl cAPP and slth cAPP, respectively, form a tetramer and a dimer. The pH dependence and pKa values of th-sl cAPP are almost identical to those of Streptomyces costaricanus. In contrast, sl-th cAPP shows curves and pKa values that resemble those of Streptomyces lividans isoform II | Streptomyces murinus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.51 | - |
L-Ala-L-Pro-4-nitroanilide | chimera th-sl cAPP, pH 7.0, 30°C | Streptomyces lividans | |
0.51 | - |
L-Ala-L-Pro-4-nitroanilide | chimera th-sl cAPP, pH 7.0, 30°C | Streptomyces murinus | |
1.4 | - |
L-Ala-L-Pro-4-nitroanilide | recombinant enzyme, pH 7.4, 30°C | Streptomyces lividans | |
1.5 | - |
L-Ala-L-Pro-4-nitroanilide | native enzyme, pH 7.4, 30°C | Streptomyces murinus | |
1.7 | - |
L-Ala-L-Pro-4-nitroanilide | recombinant enzyme, pH 7.4, 30°C | Streptomyces murinus | |
16.9 | - |
L-Ala-L-Pro-4-nitroanilide | chimera sl-th cAPP, pH 8.2, 30°C | Streptomyces lividans | |
16.9 | - |
L-Ala-L-Pro-4-nitroanilide | chimera sl-th cAPP, pH 8.2, 30°C | Streptomyces murinus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | activation | Streptomyces murinus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
4 * 55000, SDS-PAGE | Streptomyces murinus |
220000 | - |
gel filtration | Streptomyces murinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces lividans | - |
isoform APP II | - |
Streptomyces murinus | A9E3K0 | - |
- |
Streptomyces murinus TH-4 | A9E3K0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala-L-Pro-4-nitroanilide + H2O | best substrate | Streptomyces lividans | L-Ala + L-Pro-4-nitroanilide | - |
? | |
L-Ala-L-Pro-4-nitroanilide + H2O | best substrate | Streptomyces murinus | L-Ala + L-Pro-4-nitroanilide | - |
? | |
L-Ala-L-Pro-4-nitroanilide + H2O | best substrate | Streptomyces murinus TH-4 | L-Ala + L-Pro-4-nitroanilide | - |
? | |
L-Met-L-Pro + H2O | - |
Streptomyces murinus | L-Met + L-Pro | - |
? | |
L-Met-L-Pro + H2O | - |
Streptomyces murinus TH-4 | L-Met + L-Pro | - |
? | |
L-Met-L-Pro-Gly + H2O | - |
Streptomyces murinus | L-Met + L-Pro-Gly | - |
? | |
L-Met-L-Pro-Gly + H2O | - |
Streptomyces murinus TH-4 | L-Met + L-Pro-Gly | - |
? | |
L-Phe-L-Pro-Gly + H2O | - |
Streptomyces murinus | L-Phe + L-Pro-Gly | - |
? | |
L-Phe-L-Pro-Gly + H2O | - |
Streptomyces murinus TH-4 | L-Phe + L-Pro-Gly | - |
? | |
L-Phe-L-Pro-L-Ala + H2O | - |
Streptomyces murinus | L-Phe + L-Pro-L-Ala | - |
? | |
L-Phe-L-Pro-L-Ala + H2O | - |
Streptomyces murinus TH-4 | L-Phe + L-Pro-L-Ala | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 55000, SDS-PAGE | Streptomyces murinus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 40 | - |
Streptomyces murinus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | - |
stable up to | Streptomyces murinus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
L-Ala-L-Pro-4-nitroanilide | chimera th-sl cAPP, pH 7.0, 30°C | Streptomyces lividans | |
1.5 | - |
L-Ala-L-Pro-4-nitroanilide | chimera th-sl cAPP, pH 7.0, 30°C | Streptomyces murinus | |
6 | - |
L-Ala-L-Pro-4-nitroanilide | chimera sl-th cAPP, pH 8.2, 30°C | Streptomyces lividans | |
6 | - |
L-Ala-L-Pro-4-nitroanilide | chimera sl-th cAPP, pH 8.2, 30°C | Streptomyces murinus | |
45.7 | - |
L-Ala-L-Pro-4-nitroanilide | recombinant enzyme, pH 7.4, 30°C | Streptomyces lividans | |
398 | - |
L-Ala-L-Pro-4-nitroanilide | recombinant enzyme, pH 7.4, 30°C | Streptomyces murinus | |
487 | - |
L-Ala-L-Pro-4-nitroanilide | native enzyme, pH 7.4, 30°C | Streptomyces murinus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | - |
Streptomyces murinus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Streptomyces murinus |