3.4.11.20: aminopeptidase Ey

This is an abbreviated version!
For detailed information about aminopeptidase Ey, go to the full flat file.

Word Map on EC 3.4.11.20

3.4.11.20

plasmodium

falciparum

antimalarial

aminopeptidases

bestatin

hemoglobin

dipeptide

malarial

subsite

metalloaminopeptidases

yolk

hydroxamic

gallus

leucyl

exopeptidases

domesticus

Reaction

differs from other aminopeptidases in broad specificity for amino acids in the P1 position and the ability to hydrolyse peptides of four or five residues that contain Pro in the P1' position =

Synonyms

aminopeptidase M17, aminopeptidase N, ANPEP, APN1, Leucyl aminopeptidase, M1 aminopeptidase, M1 metalloaminopeptidase, M1-family aminopeptidase, M17 aminopeptidase, M17 leucyl aminopeptidase, malaria M1 metalloaminopeptidase, metallo-aminopeptidase M1, Pf-LAP, PfA-M1, PfA-M1 aminopeptidase, PfA-M17, Xac2987

ECTree

3 Hydrolases

3.4 Acting on peptide bonds (peptidases)

3.4.11 Aminopeptidases

3.4.11.20 aminopeptidase Ey

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Results	in table
5	AA Sequence
1	CAS Registry Number
11	Cloned(Commentary)
4	Crystallization (Commentary)
2	Expression
3	General Information
37	IC50 Value
67	Inhibitors
37	kcat/KM [mM/s]
25	Ki Value [mM]
40	KM Value [mM]
7	Localization
23	Metals/Ions
11	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
28	Organism
5	pH Optimum
2	pH Range
1	pH Stability
2	pI Value
3	Posttranslational Modification
2	Protein Variants
9	Purification (Commentary)
2	Reaction
1	Reaction Type
21	Reference
7	Source Tissue
2	Specific Activity [micromol/min/mg]
72	Substrates and Products (Substrate)
9	Subunits
21	Synonyms
3	Temperature Stability [°C]
39	Turnover Number [1/s]

Purification

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Purification on EC 3.4.11.20 - aminopeptidase Ey

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2 entries

amino acid residue 192-1085 as His-tagged protein: immobilized metal ion affinity chromatography (Ni2+), His-tag removed, gel filtration

Plasmodium falciparum

Q8IEK1

717834

enzyme form from egg yolk plasma fraction and from granule fraction

Gallus gallus

95176

from a modified parasite line expressing an enzyme with a C-terminal hemagglutinin tag: ion exchange chromatography, gel filtration

Plasmodium falciparum

Q8IEK1

717834

Hi-Trap Ni2+-chelating column chromatography

Xanthomonas axonopodis

682660

immobilized metal ion affinity chromatography (Ni2+), gel filtration

Plasmodium falciparum

Q8IL11

718321

p68 form, immobilized metal ion affinity chromatography (Ni2+)

Plasmodium falciparum

to homogeneity