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Literature summary for 3.4.11.20 extracted from

  • Ichishima, E.; Yamagata, Y.; Chiba, H.; Sawaguchi, K.; Tanaka, T.
    Soluble and bound forms of aminopeptidase in hen's egg yolk (1989), Agric. Biol. Chem., 53, 1867-1872.
No PubMed abstract available
Search for more literature for 3.4.11.20

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Gallus gallus
bestatin
-
 Gallus gallus
EDTA
-
 Gallus gallus
OF 4949-II substance produced by Penicillium rugulosum, CAS: [93375-50-9] Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 Leu 4-methylcoumarin 7-amide plasma enzyme Gallus gallus
0.8
-
 Leu 4-methylcoumarin 7-amide granule enzyme Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm low activity Gallus gallus 5737
-
cytoplasm 95% of the total activity Gallus gallus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ reactivates 1,10-phenanthroline inactivated apoenzyme Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
360000
-
 plasma enzyme, gel filtration Gallus gallus
700000
-
 granule enzyme, gel filtration Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
 hen
-

Purification (Commentary)

Purification (Comment) Organism
enzyme form from egg yolk plasma fraction and from granule fraction Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
egg yolk
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Leu 4-methylcoumarin 7-amide + H2O
-
 Gallus gallus ?
-
 ?
Leu 4-nitroanilide + H2O
-
 Gallus gallus ?
-
 ?
Lys-Ala-Met-Cys-Ala + H2O
-
 Gallus gallus ?
-
 ?
Lys-Phe-Ile-Gly-Leu-Met-NH2 + H2O eledoisin-related peptide, lysine, phenylalanine, isoleucine, glycine and leucine are liberated from the aminoterminus, methionine is not released from Met-NH2 of the final product Gallus gallus ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 10 min, pH 7.5, plasma enzyme stable Gallus gallus
60
-
 10 min, pH 7.5, 20% loss of plasma enzyme activity, activation of granule enzyme Gallus gallus
70
-
 10 min, pH 7.5, complete loss of activity of plasma enzyme, 60% loss of activity of granule enzyme Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 plasma enzyme Gallus gallus
7.8
-
 granule enzyme Gallus gallus