Cloned (Comment) | Organism |
---|---|
- |
Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | amino acid residue 192-1085 expressed as His-tagged protein in Escherichia coli BL21(DE3) Rosetta2 | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bestatin | - |
Plasmodium falciparum | |
bestatin methyl ester | - |
Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
Met-Phe | recombinant protein, pH 7.5, 37°C | Plasmodium falciparum | |
0.065 | - |
Met-Phe | recombinant protein, pH 5.5, 37°C | Plasmodium falciparum | |
0.086 | - |
L-arginyl-2-naphthylamide | p68/p35 complex, pH 7.5, 37°C | Plasmodium falciparum | |
0.11 | - |
L-arginyl-2-naphthylamide | p120, pH 7.5, 37°C | Plasmodium falciparum | |
0.13 | - |
Leu-Leu | recombinant protein, pH 7.5, 37°C | Plasmodium falciparum | |
0.17 | - |
L-arginyl-2-naphthylamide | recombinant protein, pH 7.5, 37°C | Plasmodium falciparum | |
0.21 | - |
L-alanyl-2-naphthylamide | recombinant protein, pH 7.5, 37°C | Plasmodium falciparum | |
0.38 | - |
Met-Phe | recombinant protein, pH 5.0, 37°C | Plasmodium falciparum | |
0.41 | - |
Leu-Leu | recombinant protein, pH 5.5, 37°C | Plasmodium falciparum | |
1.1 | - |
L-arginyl-2-naphthylamide | recombinant protein, pH 5.5, 37°C | Plasmodium falciparum | |
1.2 | - |
Leu-Leu | recombinant protein, pH 5.0, 37°C | Plasmodium falciparum | |
4.4 | - |
L-alanyl-2-naphthylamide | recombinant protein, pH 5.5, 37°C | Plasmodium falciparum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | intraerythroytic parasite | Plasmodium falciparum | 5634 | - |
vacuole | food vacuole of intraerythroytic parasite | Plasmodium falciparum | 5773 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
x * 120000, x * complex of 68000 and 35000 (generated by proteolytic cleveage within the loop sequence containing Leu-796 without N-terminal extension (residue 1-194)) | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q8IEK1 | - |
- |
Purification (Comment) | Organism |
---|---|
amino acid residue 192-1085 as His-tagged protein: immobilized metal ion affinity chromatography (Ni2+), His-tag removed, gel filtration | Plasmodium falciparum |
from a modified parasite line expressing an enzyme with a C-terminal hemagglutinin tag: ion exchange chromatography, gel filtration | Plasmodium falciparum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
trophozoite | - |
Plasmodium falciparum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanyl-2-naphthylamide + H2O | - |
Plasmodium falciparum | L-alanine + 2-naphthylamine | - |
? | |
L-arginyl-2-naphthylamide + H2O | - |
Plasmodium falciparum | L-arginine + 2-naphthylamine | - |
? | |
Leu-Leu + H2O | - |
Plasmodium falciparum | 2 L-Leu | - |
? | |
Met-Phe + H2O | - |
Plasmodium falciparum | L-Met + Phe | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 120000, x * complex of 68000 and 35000 (generated by proteolytic cleveage within the loop sequence containing Leu-796 without N-terminal extension (residue 1-194)) | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
M1-family aminopeptidase | - |
Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
Leu-Leu | recombinant protein, pH 5.0, 37°C | Plasmodium falciparum | |
3 | 6 | L-alanyl-2-naphthylamide | recombinant protein, pH 7.5, 37°C | Plasmodium falciparum | |
5.9 | - |
Met-Phe | recombinant protein, pH 7.5, 37°C | Plasmodium falciparum | |
7.4 | - |
Met-Phe | recombinant protein, pH 5.0, 37°C | Plasmodium falciparum | |
7.5 | - |
Met-Phe | recombinant protein, pH 5.5, 37°C | Plasmodium falciparum | |
7.9 | - |
Leu-Leu | recombinant protein, pH 5.5, 37°C | Plasmodium falciparum | |
9.3 | - |
L-arginyl-2-naphthylamide | p120, pH 7.5, 37°C | Plasmodium falciparum | |
9.4 | - |
L-arginyl-2-naphthylamide | recombinant protein, pH 5.5, 37°C | Plasmodium falciparum | |
11 | - |
L-arginyl-2-naphthylamide | p68/p35 complex, pH 7.5, 37°C | Plasmodium falciparum | |
12 | - |
Leu-Leu | recombinant protein, pH 7.5, 37°C | Plasmodium falciparum | |
16 | - |
L-alanyl-2-naphthylamide | recombinant protein, pH 5.5, 37°C | Plasmodium falciparum | |
21 | - |
L-arginyl-2-naphthylamide | recombinant protein, pH 7.5, 37°C | Plasmodium falciparum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | - |
Plasmodium falciparum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | at least 90% activity after 60 min | Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00012 | - |
bestatin | pH 7.5, 37°C | Plasmodium falciparum | |
0.00048 | - |
bestatin methyl ester | pH 7.5, 37°C | Plasmodium falciparum | |
0.00048 | - |
bestatin methyl ester | pH 5.5, 37°C | Plasmodium falciparum | |
0.0038 | - |
bestatin | pH 5.5, 37°C | Plasmodium falciparum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
Leu-Leu | recombinant protein, pH 5.0, 37°C | Plasmodium falciparum | |
3.7 | - |
L-alanyl-2-naphthylamide | recombinant protein, pH 5.5, 37°C | Plasmodium falciparum | |
8.7 | - |
L-arginyl-2-naphthylamide | recombinant protein, pH 5.5, 37°C | Plasmodium falciparum | |
19 | - |
Met-Phe | recombinant protein, pH 5.0, 37°C | Plasmodium falciparum | |
20 | - |
Leu-Leu | recombinant protein, pH 5.5, 37°C | Plasmodium falciparum | |
85 | - |
L-arginyl-2-naphthylamide | p120, pH 7.5, 37°C | Plasmodium falciparum | |
97 | - |
Leu-Leu | recombinant protein, pH 7.5, 37°C | Plasmodium falciparum | |
120 | - |
Met-Phe | recombinant protein, pH 5.5, 37°C | Plasmodium falciparum | |
120 | - |
L-arginyl-2-naphthylamide | recombinant protein, pH 7.5, 37°C | Plasmodium falciparum | |
130 | - |
L-arginyl-2-naphthylamide | p68/p35 complex, pH 7.5, 37°C | Plasmodium falciparum | |
170 | - |
L-alanyl-2-naphthylamide | recombinant protein, pH 7.5, 37°C | Plasmodium falciparum | |
270 | - |
Met-Phe | recombinant protein, pH 7.5, 37°C | Plasmodium falciparum |