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3.2.1.76: L-iduronidase

This is an abbreviated version!
For detailed information about L-iduronidase, go to the full flat file.

Word Map on EC 3.2.1.76

Reaction

O-(alpha-L-iduronic acid)-(1-4)-D-O-(alpha glucosamine 6-sulfate)-(1-4)-L-O-(alpha-L-iduronic acid)-D-O-2,5-anhydro-mannitol 6-sulfate
+
H2O
=
D-O-(alpha glucosamine 6-sulfate)-(1-4)-L-O-(alpha-L-iduronic acid)-D-O-2,5-anhydro-mannitol 6-sulfate
 +
alpha-L-iduronic acid

Synonyms

Aldurazyme, alpha-IDUA, alpha-iduronidase, alpha-L-iduronidase, alpha-L-iduronidase A, glycosaminoglycan alpha-L-iduronohydrolase, hIDUA, IDU, IDUA, iduronidase, laronidase, More, rIDUA_RLT

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.76 L-iduronidase

Cloned

Cloned on EC 3.2.1.76 - L-iduronidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
commercially available recombinant human iduronidase (Aldurazyme) produced in Chinese-hamster-ovary cells
-
expressed in Arabidopsis thaliana complex-glycan-deficient mutants
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expressed in Brassica rapa hairy roots
expressed in CHO cells
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expressed in CHO-K1 cells
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expressed in Mus musculus brain
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expressed in Mus musculus liver, spleen, kidney, heart and lung
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expressed in Nicotiana tabacum leaves
expressed in Rattus norvegicus primary neurons using lentivirus vectors
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expression in CHO-K1 cells
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expression in Cos-1 cells
expression in retroviral transduced fibroblasts
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expression of IDUA with and without a C-terminal endoplasmic reticulum-retention sequence SEKDEL, in seeds of Brassica napus and Nicotiana tabacum under the control of regulatory 5'-, signal-peptide-encoding-, and 3'-sequences from the arcelin 5-I gene of Phaseolus vulgaris
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expression of wild-type enzyme and enzyme in fusion with the human receptor-associated protein, RAP, in LRP1-null CHO-K1 cells
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gene IDUA, screening and genotyping of nonsense, 4 missense, 1 deletion, and 2 splice site intron mutations in 10 MPS I patients, development and evaluation of a dHPLC screening method, overview
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genotyping of different mucopolysaccharidosis type I patient cell lines, overview
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human-mouse somatic cell hybrids
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IDUA cloning in Escherichia coli strain BL21, and overexpression in transgenic Nicotiana tabacum BY-2 cells, establishing and evaluation of the expression system, overview. A plant signal peptide is essential for proper expression and secretion of the glycosylated recombinant hIDUA into the cultured media of transgenic BY-2 cells, transfection by Agrobacterium tumefaciens, strain LBA4404
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IDUA gene, determination of mutant sequences from mucopolysaccharidosis type I enzymes, genotyping, expression of wild-type and mutant enzymes in CHO cells
incorporated via mannose 6-phosphate receptors into human fibroblasts from a patient with MPS I (F17) or a healthy subject (F592), into cultured mouse osteoblasts (MC3T3-E1) or cultured mouse fibroblasts (F665)
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recombinant expression in baby hamster kidney cells
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