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3.2.1.76: L-iduronidase

This is an abbreviated version!
For detailed information about L-iduronidase, go to the full flat file.

Word Map on EC 3.2.1.76

Reaction

O-(alpha-L-iduronic acid)-(1-4)-D-O-(alpha glucosamine 6-sulfate)-(1-4)-L-O-(alpha-L-iduronic acid)-D-O-2,5-anhydro-mannitol 6-sulfate
+
H2O
=
D-O-(alpha glucosamine 6-sulfate)-(1-4)-L-O-(alpha-L-iduronic acid)-D-O-2,5-anhydro-mannitol 6-sulfate
 +
alpha-L-iduronic acid

Synonyms

Aldurazyme, alpha-IDUA, alpha-iduronidase, alpha-L-iduronidase, alpha-L-iduronidase A, glycosaminoglycan alpha-L-iduronohydrolase, hIDUA, IDU, IDUA, iduronidase, laronidase, More, rIDUA_RLT

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.76 L-iduronidase

Specific Activity

Specific Activity on EC 3.2.1.76 - L-iduronidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000014 - 0.000027
mucopolysaccharidosis type I mutant enzymes, immunocapture-enriched from skin fibroblast
0.000005 - 0.00011
-
activity levels from the same specimens of heterozygous mice vary greatly with different substrate concentrations of 4-methylumbelliferyl-alpha-L-iduronide, comparison of activities in livers at 15 different concentrations, overview. pH and temperature not specified in the publication
0.00003 - 0.000093
wild-type enzyme, immunocapture-enriched from skin fibroblast
0.0006
recombinant wild-type enzyme, CHO cells
0.0029
-
recombinant enzyme, using 4-methylumbelliferyl-alpha-L-iduronide as a substrate
0.0033 - 0.0053
-
wild-type cell lines, pH not specified in the publication, 37°C
0.018
-
native enzyme, using 4-methylumbelliferyl-alpha-L-iduronide as a substrate
0.03
-
recombinant enzyme, using 4-methylumbelliferyl-alpha-L-iduronide as a substrate
0.3
recombinant mutant G265R, CHO cells
0.6
recombinant wild-type enzyme, CHO cells
1.5
recombinant mutant A79V, CHO cells
109
-
membrane associated form
126000
-
-
16
-
phenyl alpha-L-iduronide as substrate
2.08
-
-
2.1
recombinant mutant L238Q, CHO cells
2.9
recombinant mutant F602I, CHO cells
270
-
anhydromannitol iduronide as substrate
32
-
purified recombinant enzyme expressed from Brassica napus seeds
34.9
-
-
39.7
-
purified recombinant enzyme expressed from Nicotiana tabacum seeds
4.8
recombinant mutant H82Q, CHO cells
5000 - 10000
-
-
6.7
recombinant mutant S423R, CHO cells
87.5
-
soluble form
additional information
-
specific activity in healthy individuals was found to be higher than specific activity in mucopolysaccharidosis patients group I but similar with mucopolysaccharidosis patients group II