3.2.1.58: glucan 1,3-beta-glucosidase This is an abbreviated version! For detailed information about glucan 1,3-beta-glucosidase, go to the full flat file .
Reaction
(Glcbeta(1-3))n +
H2O =
(Glcbeta(1-3))n-1 +
alpha-D-glucopyranose
Synonyms 1,3-beta-D-glucanohydrolase, AkLam33, ALAM, beta-(1,3)(1,3)-glucanase, beta-(1,3)-glucanase, beta-1,3-exoglucanase, beta-1,3-glucan exo-hydrolase, beta-1,3-glucanase, BGL1, Bglu3B, BGN3.2, BGN3.4, CC1G_06564, CC1G_07313, Celf_3321, ChinLam, Exg, Exg1, EXG2, ExgA, ExgP, exo (13)-beta-glucanase, exo-1,3-beta-D-glucanase, exo-1,3-beta-glucanase, Exo-1,3-beta-glucanase I/II, exo-1,3-beta-glucosidase, Exo-beta 1,3 glucanase, exo-beta-(1,3)-glucanase, exo-beta-(13)-D-glucanase, exo-beta-(13)-glucanohydrolase, exo-beta-1,3-D-glucanase, exo-beta-1,3-glucanase, exo-beta-glucanase, Exo1, Glu1, Glu17A, glucan (1-->3)-beta-glucosidase, GP29, Lam55, Lam55A, lamC, laminarinase, More, panomycocin, PiEXO, tag83, Xog1
ECTree
Temperature Stability
Temperature Stability on EC 3.2.1.58 - glucan 1,3-beta-glucosidase
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22
-
pH 3.5-7.5, stable for 24 h
30 - 40
-
purified enzyme, highly stable between for 1 h in citrate-phosphate buffer at pH 7.0
35
Fungi imperfecti
-
stable below 35°C
40
purified recombinant enzyme, stable up to
50 - 70
Thermochaetoides thermophila
the enzyme is thermostable at 50°C but retains about 40% of its activity after incubation at 60°C for10 min (half-life of the enzyme at 60°C is about 8 min). Most of the enzyme activity is lost at above 70°C after 10 min
52
-
stable at 52°C for 20 min
55
-
enzyme I, stable below 55°C
70
Thermochaetoides thermophila
-
half-life: 21.5 min
80
Thermochaetoides thermophila
-
half-life: 5 min
30
-
stable below
30
-
looses activity at higher temperatures
45
-
stable below 45°C
45
-
stable for 45 min, underglycosylated form after 45 min 35% loss of activity
45
-
90 min, 85% residual activity
50
-
enzyme II and III, stable below 50°C
50
-
the enzyme retains more than 80% of its original activity after 30 min of incubation at 50°C
50
-
stable up to, on heating for 30 min
50
Thermochaetoides thermophila
-
stable at
50
-
90 min, 57% residual activity
60
-
30 min, inactivated above
60
Thermochaetoides thermophila
-
1 h, enzyme retains 90% of its activity
60
-
after 15 min 100% loss of activity
65
-
half-life 18.3 h
65
Thermochaetoides thermophila
-
half-life: 55 min