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3.2.1.58: glucan 1,3-beta-glucosidase

This is an abbreviated version!
For detailed information about glucan 1,3-beta-glucosidase, go to the full flat file.

Word Map on EC 3.2.1.58

Reaction

(Glcbeta(1-3))n
+
H2O
=
(Glcbeta(1-3))n-1
+
alpha-D-glucopyranose

Synonyms

1,3-beta-D-glucanohydrolase, AkLam33, ALAM, beta-(1,3)(1,3)-glucanase, beta-(1,3)-glucanase, beta-1,3-exoglucanase, beta-1,3-glucan exo-hydrolase, beta-1,3-glucanase, BGL1, Bglu3B, BGN3.2, BGN3.4, CC1G_06564, CC1G_07313, Celf_3321, ChinLam, Exg, Exg1, EXG2, ExgA, ExgP, exo (13)-beta-glucanase, exo-1,3-beta-D-glucanase, exo-1,3-beta-glucanase, Exo-1,3-beta-glucanase I/II, exo-1,3-beta-glucosidase, Exo-beta 1,3 glucanase, exo-beta-(1,3)-glucanase, exo-beta-(13)-D-glucanase, exo-beta-(13)-glucanohydrolase, exo-beta-1,3-D-glucanase, exo-beta-1,3-glucanase, exo-beta-glucanase, Exo1, Glu1, Glu17A, glucan (1-->3)-beta-glucosidase, GP29, Lam55, Lam55A, lamC, laminarinase, More, panomycocin, PiEXO, tag83, Xog1

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.58 glucan 1,3-beta-glucosidase

Purification

Purification on EC 3.2.1.58 - glucan 1,3-beta-glucosidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 mg pure protein per 10 liters culture
-
432.7fold
-
enzyme I 22fold, enzyme II 25fold, enzyme III 28fold
-
expressed in Escherichia coli
-
isoenzyme I: 47fold, isoenzyme II: 128fold
native enzyme 152fold by ammonium sulfate fractionation, ion exchange chromatography and gel filtration
-
native enzyme 28fold by ammonium sulfate fractionation, and several steps of cation exchange chromatography
-
Ni-NTA column chromatography
-
partial purification
-
recombinant ExgP from Aspergillus oryzae culture supernatant by anion exchange and hydrophobic interaction chromatography, and gel filtration
-
recombinant His6-tagged enzyme 2.1fold from Yarrowia lipolytica strain Po1h to homogeneity
soluble native enzyme 115fold from strain WC91-2 to homogeneity by ultrafiltration, gel filtration, and anion exchange chromatography