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3.2.1.17: lysozyme

This is an abbreviated version!
For detailed information about lysozyme, go to the full flat file.

Word Map on EC 3.2.1.17

Reaction

N,N',N'',N'''-tetraacetylchitotetraose
+
H2O
=
N,N',N''-triacetylchitotriose
 +
N-acetyl-D-glucosamine

Synonyms

1,4-beta-N-acetylmuramidase, 1,4-beta-N-acetylmuramidase 1, 1,4-beta-N-acetylmuramidase A/C, 1,4-beta-N-acetylmuramidase M1, 1,4-beta-N-acetylmuramoylhydrolase, 1,4-N-acetylmuramidase, abLysI, AcmB, ASTL, Autolysin, BA-lysozyme, c-type lysozyme, CCLys-g, CFL, chicken-type lysozyme, cLys3, CP-1 lysin, CP-7 lysin, CP-9 lysin, CPL, DEL-I, DEL-II, DEL-III, destabilase-lysozyme, EGL, Egyptian goose egg-white lysozyme, Egyptian goose lysozyme, endolysin, EQL, equine lysozyme, fOg44 endolysin, g-lysozyme, g-type lysozyme, GEWL, globulin G, globulin G1, goose type lysozyme, Goose-type lysozyme, gp144, gp36C, GSTL, HEWL, HLysG2, hLYZ, HLZ, i-type lysozyme, iLys, iLys2, invertebrate lysozyme, invertebrate type lysozyme, L-7001, LAL, lambda lysozyme, Late protein gp15, Lys-rich lysozyme 2, lysC, LYSC-1, LysG, LysgaY, LysGL, lysi1, lysi2, Lysis protein, Lysosyme, lysozym, Lysozyme, lysozyme 1, lysozyme 1 precursor, lysozyme A, lysozyme B, lysozyme c, lysozyme c-1, lysozyme g, lysozyme g-like 2, lysozyme SP0987, lysozyme-g, LYZ, LYZ2, M1L, Mdl1, MdL2, mlD-Ds1, mlD-Ds2, mlD-Ds3, MLL-A, MLL-B, mucopeptide glucohydrolase, mucopeptide N-acetylmuramic acid hydrolase, mucopeptide N-acetylmuramoylhydrolase, muramidase, mutanolysin, MV1 lysin, N,O-diacetylmuramidase, N-acetylglucosaminidase autolysin, N-acetylmuramic hydrolase, N-acetylmuramide glycanhydrolase, n-SalC, n-SalG, OEL, OHLysG1, OHLysG2, OHLysG3, Outer wedge of baseplate protein, P13, PALysG, Peptidoglycan hydrolase, peptidoglycan n-acetylmuramic hydrolase, phage-type like lysozyme, phiKZ endolysin, Ply3626, PR1-lysozyme, Protein gp17, Protein gp19, Protein Gp25, Protein Gp5, Protein gp54, Protein gpK, SalG, SjLys, SmLysC, SSTL A, SSTL B, T4 lysozyme, T4L, TJL, transglycosylase, VpLYZ, WGL, wood duck lysozyme

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.17 lysozyme

Cloned

Cloned on EC 3.2.1.17 - lysozyme

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 different kinds of transgenic mice containing human lysozyme genomic DNA-based constructs with different signal peptide DNA are respectively established as system models
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a C-terminal hexahistidine tail Lys44 is overproduced in Escherichia coli
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a large polymannose (Man310GlcNAc2) chain-linked lysozyme is predominantly expressed in Saccharomyces cerevisiae accompanied by small amounts of a core-type oligomannose chain (Man14GlcNAc2)-linked lysozyme in the yeast medium where the extracellular pH is kept at 3.5 or above, while an oligomannose chain lysozyme is preferentially expressed in the yeast medium where the pH is less than 3
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acmB gene, AcmB devoid of its N-terminal domain, expression in Escherichia coli M15(pREP4), sequencing
amino acid Ser at the N-terminus
-
DNA and amino acid sequence determination and analysis, phylogenetic analysis
DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain BL21(DE3) and Bacillus subtilis strain WB800, subcloning in Escherichia coli strain DH5alpha
-
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree, expression of enzyme mutants in Pichia pastoris, subcloning in Escherichia coli strain JM109
expressed in Escherichia coli BL21 Star cells
Tequatrovirus T4
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Rosetta (DE3) cells
-
expressed in Gallus gallus eggs
-
expressed in Pichia pastoris
expression in Escherichia coli
expression in goat at 67% of human breast milk
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expression in human cell lines HeLa and MCF-7
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expression in Pichia pastoris
expression in Saccharomyces cerevisiae
expression of 253 C-terminal residues in Escherichia coli
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expression of enzyme mutants in Escherichia coli strain BL21(DE3) in inclusion bodies
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expression of recombinant protein in Saccharomyces cerevisiae
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expression of the enzyme in transgenic cattle, Bos taurus, as secreted enzyme in milk using the pBC2-HLY-NEOR transgene vector
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expression of wild-type and engineered enzyme in Saccharomyces cerevisiae
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gene abLysI, DNA and amino acid sequence determination and analysis, sequence comparison and phylogeny analysis, enzyme overexpression in Escherichia coli strain BL21(DE3)
gene hlyz, cloning in Escherichia coli strain JM105, optimization and evaluation of an efficient expression system capable of producing folded, soluble and functional human lysozyme in Escherichia coli, simultaneously co-expression of multiple protein folding chaperones as well as harnessing of the lysozyme inhibitory protein, Ivy, overview
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gene RpLysPh, DNA and amino acid sequence determination and analysis, phylogenetic analysis and real-time quantitative RT-PCR expression analysis
genomic library construction, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, expression in Spodoptera frugiperda Sf9 cells using the baculovrisu transfection system via host Escherichia coli strain coli DH10Bac
into the pQE-30 vector for expression in Escherichia coli cells
Phikzvirus phiKZ
isozyme OHLysG1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis
-
isozyme OHLysG2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis
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isozyme OHLysG3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis
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lysozyme is amplified from bacteriophage lambda cDNA, recombinant enzyme expression in Escherichia coli strain DH5alpha
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overexpression in Escherichia coli
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overexpression of GST-tagged enzyme in inclusion body in Escherichia coli with induction of the fusion protein
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ply3626 gene, expression in Escherichia coli JM109
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recombinant enzyme expression in Spodoptera frugiperda cells via baculovirus transfection method
recombinant enzyme expression in sugarcane stalks, Saccharum spp. hybrid, process evaluation and optimization, overview
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recombinant expression of the mature peptide-coding region in Pichia pastoris strain GS115
recombinant expression of wild-type and mutant MdL1 and MdL2 in Pichia pastoris
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sequence comparisons, phylogenetic tree
sequence comparisons, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
T4 lysozyme is cloned into the yeast expression vector pPIC9K under the control of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase promoter and expressed at high level in Hansenula polymorpha strain A16, the enzyme is secreted
Tequatrovirus T4
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Tapes japonica lysozyme (TjL) is recombinantly produced using Pichia pastoris YJT46
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the fragment is expressed in both BL21 (DE3) and B834 (DE3) Escherichia coli cells
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the gene coding for lysozyme in banana prawn (Fenneropenaeus merguiensis) is cloned, sequenced and expressed in pET-32a vector (Escherichia coli)
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the gene encoding the Bacillus anthracis GH25 enzyme, BaGH25c, is expressed in Escherichia coli
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the His-tagged protein Lys411H is expressed in Escherichia coli mostly in inclusion bodies
the mature peptide coding region is heterologously expressed in Escherichia coli
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two serine-rich heptapeptides, Ser-Ser-Ser-Lys-Ser-Ser-Ser (S6K) and Ser-Ser-Ser-Ser-Ser-Ser-Ser (S7) are fused to the C-terminus of chicken lysozyme by genetic modification. The cDNAs of S6K-lysozyme and S7-lysozyme are inserted into the expression vector of Pichia pastoris and secreted in the yeast cultivation medium. The secretion amounts of S6K-lysozyme and S7-lysozyme are about 60% of that of wild-type lysozyme
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